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PMID: 6983692 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A T15-idiotype-positive T suppressor hybridoma does not use the T15 VH gene segment.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 22 ·1982-11-00 ·Pages 6984-8

Nakanishi K, Sugimura K, Yaoita Y, Maeda K, Kashiwamura S, Honjo T, Kishimoto T

Abstract

The T suppressive factor (TsF) released from a T15-idiotype-positive phosphocholine (PCho)-specific T hybridoma, F18-3-4, which was formed by fusion between BALB/c T cells and BW5147 thymoma, was immunochemically characterized. TsF inhibited the in vitro induction of both IgE and IgG1 antibody responses of 2,4-dinitrophenyl keyhole limpet hemocyanin (DNP-KLH)-primed spleen cells in the presence of PCho-KLH-DNP. TsF had the ability to bind to PCho determinants and possessed T15 idiotype determinants as well as Iad products. However, we were unable to detect either the rearrangement of the T15 VH gene or the presence of T15 VH gene transcripts in hybridomas by DNA and RNA blot hybridization analyses with the T15 VH DNA probe.

MeSH Terms

Animals Antibody Formation Binding Sites, Antibody/genetics Genes Hybridomas/immunology Immunoglobulin Idiotypes/genetics Immunoglobulin Variable Region/genetics Immunosuppression Therapy Mice Mice, Inbred BALB C Nucleic Acid Hybridization T-Lymphocytes/immunology

Chemicals

Immunoglobulin Idiotypes Immunoglobulin Variable Region

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Nakanishi K

Sugimura K

Yaoita Y

Maeda K

Kashiwamura S

Honjo T

Kishimoto T

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.22.6984

Published

1982-11-00

Pages

6984-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC347259

Subset

External Links

PubMed source DOI: 10.1073/pnas.79.22.6984 PMC Full Text View journal details

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A T15-idiotype-positive T suppressor hybridoma does not use the T15 VH gene segment.

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