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PMID: 6988386 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Yeast mutants auxotrophic for choline or ethanolamine.

Journal of bacteriology ·Vol. 141 ·No. 2 ·1980-02-00 ·Pages 558-64

Atkinson KD, Jensen B, Kolat AI, Storm EM, Henry SA, Fogel S

Abstract

Three mutants of the yeast Saccharomyces cerevisiae which require exogenous ethanolamine or choline were isolated. The mutants map to a single locus (cho1) on chromosome V. The lipid composition suggests that cho1 mutants do not synthesize phosphatidylserine under any growth conditions. If phosphatidylethanolamine or phosphatidylcholine, which are usually derived from phosphatidylserine, were synthesized from exogenous ethanolamine or choline, the mutants grew and divided relatively normally. However, mitochondrial abnormalities were evident even when ethanolamine and choline were supplied. Diploids homozygous for the cho1 mutation were defective in sporulation. Growth on nonfermentable carbon sources was slow, and a high proportion of respiratory-deficient (petite) cells were generated in cho1 cultures.

MeSH Terms
Choline/metabolism Ethanolamines/metabolism Inositol/metabolism Mutation Phosphatidylserines/genetics Phospholipids/biosynthesis Saccharomyces cerevisiae/genetics,growth & development,metabolism
Chemicals
Ethanolamines Phosphatidylserines Phospholipids Inositol Choline
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Atkinson K D
Jensen B
Kolat A I
Storm E M
Henry S A
Fogel S
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31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.141.2.558-564.1980
Published
1980-02-00
Pages
558-64
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC293659
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.141.2.558-564.1980 PMC Full Text View journal details
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