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PMID: 7000755 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transposed LEU2 gene of Saccharomyces cerevisiae is regulated normally.

Journal of bacteriology ·Vol. 144 ·No. 2 ·1980-11-00 ·Pages 852-5

Kohlhaw GB, Hsu YP, Lemmon RD, Petes TD

Abstract

The repression of beta-isopropylmalate dehydrogenase, the LEU2 gene product, by leucine and leucine plus threonine was unaffected by the transposition of LEU2 from its original locus on chromosome III to a new locus within the ribosomal deoxyribonucleic acid gene cluster on chromosome XII. Since the expression of the LEU2 gene is probably controlled at a pretranslational level, we conclude that the recombinant plasmid used for transformation carries regulatory information in addition to LEU2 structural information.

MeSH Terms
3-Isopropylmalate Dehydrogenase Alcohol Oxidoreductases/biosynthesis,genetics DNA, Recombinant Enzyme Repression Gene Expression Regulation Genes, Regulator Leucine/pharmacology Recombination, Genetic Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA, Recombinant Alcohol Oxidoreductases 3-Isopropylmalate Dehydrogenase Leucine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kohlhaw G B
Hsu Y P
Lemmon R D
Petes T D
References (12)
12 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.144.2.852-855.1980
Published
1980-11-00
Pages
852-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294742
Subset
IM
Grants
NCI NIH HHS · CA-14599 · United States
NIGMS NIH HHS · GM15102 · United States
NIGMS NIH HHS · GM24110 · United States
External Links
PubMed source DOI: 10.1128/jb.144.2.852-855.1980 PMC Full Text View journal details
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