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PMID: 7000792 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells.

The Journal of cell biology ·Vol. 87 ·No. 2 Pt 1 ·1980-11-00 ·Pages 326-35

Cande WZ

Abstract

PtK1 cells lysed late in cell division in a medium containing the nonionic detergent Brij 58 and polyethylene glycol with continue to undergo cleavage after lysis. Maintenance of cleavage after lysis is dependent on the composition of the lysis medium; the pH must be around neutrality, MgATP must be present, and the free Ca++ concentration should be 1 microM for optimal constriction to occur. Cleavage can be stopped and reinitiated by raising and lowering the Ca++ levels in the lysis medium. Cleavage in the permeabilized cell is blocked by addition of phalloidin, cytochalasin B, and N-ethylmaleimide-modified myosin subfragment-1 to the lysis medium. This represents the first cell model system for studying cleavage since the pioneering studies of Hoffman-Berling in 1954.

MeSH Terms
Adenosine Triphosphate/physiology Animals Calcium/physiology Cell Division/drug effects Cell Membrane Permeability Cells, Cultured Cytochalasin B/pharmacology Detergents Ethylmaleimide/pharmacology Hydrogen-Ion Concentration Models, Biological Myosin Subfragments/pharmacology Phalloidine/pharmacology
Chemicals
Detergents Myosin Subfragments Phalloidine Cytochalasin B Adenosine Triphosphate Ethylmaleimide Calcium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cande W Z
References (32)
32 references, click to expand
  1. Prevention of the cortical reaction in fertilized sea urchin eggs by injection of calcium-chelating ligands.
    Biochim Biophys Acta. 1978 Jul 17;541(4):459-66 PMID: 96872
  2. Effect of microinjected N-ethylmaleimide-modified heavy meromyosin on cell division in amphibian eggs.
    J Cell Biol. 1980 Sep;86(3):858-65 PMID: 6997323
  3. Intracellular calcium and cell cleavage in early embryos of Xenopus laevis.
    J Cell Biol. 1972 May;53(2):579-81 PMID: 5063471
  4. [Glycerin water extracted telophase cells as a model of cytokinesis].
    Biochim Biophys Acta. 1954 Nov;15(3):332-9 PMID: 13230071
  5. The effect of myosin antibody on the division of starfish blastomeres.
    J Cell Biol. 1977 Jul;74(1):251-63 PMID: 141455
  6. THE DEPENDENCE OF CONTRACTION AND RELAXATION OF MUSCLE FIBRES FROM THE CRAB MAIA SQUINADO ON THE INTERNAL CONCENTRATION OF FREE CALCIUM IONS.
    Biochim Biophys Acta. 1964 May 25;79:581-91 PMID: 14179458
  7. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5613-7 PMID: 341163
  8. Experiments concerning the cleavage furrow in invertebrate eggs.
    J Exp Zool. 1966 Feb;161(1):1-8 PMID: 4380557
  9. Regulation of the actin-myosin interaction in vertebrate smooth muscle: activation via a myosin light-chain kinase and the effect of tropomyosin.
    J Mol Biol. 1977 Jun 5;112(4):559-76 PMID: 195061
  10. Tension generation by threads of contractile proteins.
    J Gen Physiol. 1977 Jan;69(1):37-55 PMID: 137958
  11. [LOCAL CONTRACTION AS A RESULT OF CYTOPLASMIC CLEAVAGE OF FIBROBLAST CELLS].
    Biochim Biophys Acta. 1964 Jan 27;79:98-101 PMID: 14114533
  12. The contractile basis of ameboid movement. VI. The solation-contraction coupling hypothesis.
    J Cell Biol. 1979 Dec;83(3):633-48 PMID: 42649
  13. GLYCERINATED SKELETAL AND SMOOTH MUSCLE: CALCIUM AND MAGNESIUM DEPENDENCE.
    Science. 1965 Mar 26;147(3665):1581-3 PMID: 14260375
  14. Cytochalasin B and the structure of actin gels.
    J Mol Biol. 1979 Nov 5;134(3):539-53 PMID: 583431
  15. Interaction of actin with phalloidin: polymerization and stabilization of F-actin.
    Biochim Biophys Acta. 1975 Aug 19;400(2):407-14 PMID: 126084
  16. Analysis of actin and microfilament-associated proteins in the mitotic spindle and cleavage furrow of PtK2 cells by immunofluorescence microscopy. A critical note.
    Exp Cell Res. 1979 Nov;124(1):93-109 PMID: 387430
  17. Actin and myosin and cell movement.
    CRC Crit Rev Biochem. 1974 Jan;2(1):1-65 PMID: 4273099
  18. [Adenosinetriphosphate as the energy substance for cell movement].
    Biochim Biophys Acta. 1954 Jun;14(2):182-94 PMID: 13172235
  19. The influence of microinjected phalloidin on locomotion, protoplasmic streaming and cytoplasmic organization in Amoeba proteus and Physarum polycephalum.
    Cytobiologie. 1978 Oct;18(1):114-31 PMID: 710672
  20. The informational role of calcium in the cytosol.
    Adv Cyclic Nucleotide Res. 1979;11:1-26 PMID: 158957
  21. Changing patterns of actin localization during cell division.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1913-6 PMID: 1098046
  22. Actin in dividing cells: contractile ring filaments bind heavy meromyosin.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1688-92 PMID: 4578441
  23. Chromosome movement in lysed mitotic cells is inhibited by vanadate.
    J Cell Biol. 1978 Nov;79(2 Pt 1):573-80 PMID: 152767
  24. N-ethylmaleimide-modified heavy meromyosin. A probe for actomyosin interactions.
    J Cell Biol. 1979 Jul;82(1):57-65 PMID: 158029
  25. The contractile ring. II. Determining its brief existence, volumetric changes, and vital role in cleaving Arbacia eggs.
    J Cell Biol. 1972 May;53(2):419-34 PMID: 5063470
  26. Cytokinesis in animal cells.
    Int Rev Cytol. 1971;31:169-213 PMID: 4400359
  27. Cytoplasmic structure and contractility in amoeboid cells.
    Int Rev Cytol. 1979;56:57-144 PMID: 37189
  28. Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells.
    J Cell Biol. 1976 Dec;71(3):848-75 PMID: 62755
  29. A fine structural analysis of cleavage induction and furrowing in the eggs of Arbacia punctulata.
    J Cell Biol. 1969 Jul;42(1):170-84 PMID: 5786981
  30. Ionophore A23187, calcium and contractility in frog eggs.
    Exp Cell Res. 1974 Jan;83(1):139-42 PMID: 4204796
  31. Alpha-actinin localization in the cleavage furrow during cytokinesis.
    J Cell Biol. 1978 Oct;79(1):268-75 PMID: 359574
  32. Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin.
    Nature. 1975 Sep 4;257(5521):54-6 PMID: 125854
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.87.2.326
Published
1980-11-00
Pages
326-35
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110752
Subset
IM
Grants
NCRR NIH HHS · BRSI507 RR0706. · United States
NIGMS NIH HHS · GM23238 · United States
External Links
PubMed source DOI: 10.1083/jcb.87.2.326 PMC Full Text View journal details
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