Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 7 ·1980-07-00 ·Pages 4147-51

Cortese R, Harland R, Melton D

Abstract

The expression of cloned tRNA genes has been studied by injecting single-stranded and double-stranded DNA templates into Xenopus oocyte nuclei. In both forms the genes are faithfully transcribed after injection. Some single-stranded DNA is converted into double-stranded DNA in the oocyte nucleus. This conversion is necessary for the expression of the injected tRNA gene: no tRNA transcription is observed when DNA synthesis is inhibited. We conclude that single-stranded DNA does not serve as a template for faithful transcription of this gene in injected oocytes.

MeSH Terms

Coliphages/genetics DNA, Bacterial/genetics DNA, Single-Stranded/genetics Escherichia coli/genetics Genes Plasmids RNA, Transfer/genetics Templates, Genetic Transcription, Genetic

Chemicals

DNA, Bacterial DNA, Single-Stranded RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cortese R

Harland R

Melton D

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.7.4147

Published

1980-07-00

Pages

4147-51

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC349787

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.7.4147 PMC Full Text View journal details

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Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.

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