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PMID: 7002911 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Deletion of the penicillin-binding protein 5 gene of Escherichia coli.

Journal of bacteriology ·Vol. 144 ·No. 3 ·1980-12-00 ·Pages 1190-2

Spratt BG

Abstract

A strain of Escherichia coli that has a deletion of the entire dacA gene has been constructed. The complete lack of penicillin-binding protein 5 in this strain establishes that the activity of this protein is not essential for the growth of E. coli.

MeSH Terms

Bacterial Proteins Carrier Proteins/physiology Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics,growth & development Genes Hexosyltransferases Muramoylpentapeptide Carboxypeptidase Mutation Penicillin-Binding Proteins Penicillins/physiology Peptidyl Transferases

Chemicals

Bacterial Proteins Carrier Proteins Penicillin-Binding Proteins Penicillins Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Spratt B G

References (12)

12 references, click to expand

Glycopeptide transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reactions.
Proc Natl Acad Sci U S A. 1966 Mar;55(3):656-63 PMID: 5329013
Purification to homogeneity and properties of two D-alanine carboxypeptidases I From Escherichia coli.
J Biol Chem. 1976 Jan 25;251(2):414-23 PMID: 1391
Identification of the major penicillin-binding proteins of Escherichia coli as D-alanine carboxypeptidase IA.
J Bacteriol. 1976 Jul;127(1):660-3 PMID: 776946
Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2976-9 PMID: 331322
Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2980-4 PMID: 331323
Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):664-8 PMID: 345275
Isolation of a mutant of Escherichia coli lacking penicillin-sensitive D-alanine carboxypeptidase IA.
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2631-5 PMID: 351612
Mutational evidence for identity of penicillin-binding protein 5 in Escherichia coli with the major D-alanine carboxypeptidase IA activity.
J Bacteriol. 1979 Jan;137(1):644-7 PMID: 368033
Linkage map of Escherichia coli K-12, edition 6.
Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
Biochemical and genetical approaches to the mechanism of action of penicillin.
Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):273-83 PMID: 6109323
Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome.
J Bacteriol. 1980 Aug;143(2):569-81 PMID: 6451612

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.144.3.1190-1192.1980

Published

1980-12-00

Pages

1190-2

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC294790

Subset

External Links

PubMed source DOI: 10.1128/jb.144.3.1190-1192.1980 PMC Full Text View journal details

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Deletion of the penicillin-binding protein 5 gene of Escherichia coli.

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