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PMID: 7003713 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels.

Science (New York, N.Y.) ·Vol. 211 ·No. 4477 ·1981-01-02 ·Pages 70-2

Glass WF, Briggs RC, Hnilica LS

Abstract

Nonhistone protein antigens resolved by electrophoresis in sodium dodecyl sulfate were identified immunochemically after being transferred to nitrocellulose. Use of antiserum to dehistonized chromatin from Novikoff hepatoma revealed numerous protein antigens specific to the chromatin of Novikoff hepatoma in comparison to that of normal rat liver.

MeSH Terms

Animals Cell Nucleus/immunology Chromosomal Proteins, Non-Histone/analysis,immunology Electrophoresis, Polyacrylamide Gel/methods Immunoenzyme Techniques Liver/analysis,ultrastructure Liver Neoplasms, Experimental/analysis,ultrastructure Male Rats

Chemicals

Chromosomal Proteins, Non-Histone

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Glass W F

Briggs R C

Hnilica L S

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.7003713

Published

1981-01-02

Pages

70-2

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NCI NIH HHS · CA-26412 · United States

NCI NIH HHS · CA-26948 · United States

NICHD NIH HHS · HD-07043-04 · United States

External Links

PubMed source DOI: 10.1126/science.7003713 View journal details

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Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels.

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