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PMID: 7004962 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Preliminary characterization of calcium binding in islet-cell plasma membranes.

Diabetologia ·Vol. 19 ·No. 5 ·1980-11-00 ·Pages 439-44

Naber SP, McDonald JM, Jarett L, McDaniel ML, Ludvigsen CW, Lacy PE

Abstract

Preliminary characterization of calcium binding was determined in a highly-enriched islet-cell plasma membrane fraction using a membrane filtration technique. Equilbrium calcium binding was specific, concentration dependent and saturable. Scatchard analysis indicated the existence of more than one class of calcium binding sites. The affinity constants and maximum binding capacities were 1.14 X 10(5) M-1 and 1.2 picomol/microgram protein and 1.17 X 10(3) M-1 and 64.8 picomol/microgram for the high and low affinity sites, respectively. Bound 45Ca2+ was dissociate from the plasma membranes in a biphasic manner in the presence of excess unlabelled calcium.

MeSH Terms
Animals Binding Sites Calcium/metabolism Calcium Radioisotopes Cell Fractionation Cell Membrane/metabolism Insulin/metabolism Insulin Secretion Islets of Langerhans/metabolism Rats
Chemicals
Calcium Radioisotopes Insulin Calcium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Naber S P
McDonald J M
Jarett L
McDaniel M L
Ludvigsen C W
Lacy P E
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31 references, click to expand
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Article Info
Journal
Diabetologia
Abbr.
Diabetologia
ISSN
0012-186X
DOI
10.1007/BF00281823
Published
1980-11-00
Pages
439-44
Language
English
Region
Germany
NLM ID
0006777
Subset
IM
Grants
NIADDK NIH HHS · AM05398 · United States
NIADDK NIH HHS · AM06181 · United States
NIADDK NIH HHS · AM11892 · United States
External Links
PubMed source DOI: 10.1007/BF00281823 View journal details
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