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PMID: 7007313 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxu operon by exuR and uxuR gene products.

Journal of bacteriology ·Vol. 145 ·No. 1 ·1981-01-00 ·Pages 211-20

Robert-Baudouy J, Portalier R, Stoeber F

Abstract

New regulatory mutants of Escherichia coli K-1 carrying alterations of the uxuR gene were isolated and characterized. In the presence of superrepressed or derepressed uxuR mutations, mannonic hydrolyase (uxuA) and oxidoreductase relationship analyses suggested that the uxuR gene product acted as a repressor in the control of uxuA-uxuB operon expression. uxuR mutations were localized near min 97, and the following gene order was established: (argH)-uxuR-uxuB-uxuA-(thr). Properties of exuR (point and deletion) mutants showed that both exuR and uxuR regulatory gene products were involved in the control of the uxuA uxuB operon. Analysis of exuR uxuR double-derepressed mutants suggested that exuR and uxuR repressors act cooperatively to repress the uxu operon.

MeSH Terms
Escherichia coli/genetics,metabolism Fructuronate Reductase/genetics Gene Expression Regulation Genes, Regulator Hexuronic Acids/metabolism Hydro-Lyases/genetics Mutation Operon Repressor Proteins/physiology Transcription Factors/physiology Uronic Acids/metabolism
Chemicals
Hexuronic Acids Repressor Proteins Transcription Factors Uronic Acids Fructuronate Reductase Hydro-Lyases mannonate dehydratase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Robert-Baudouy J
Portalier R
Stoeber F
References (24)
24 references, click to expand
  1. Control of the biosynthesis of carbamoyl phosphate in Escherichia coli.
    J Mol Biol. 1965 Nov;14(1):23-36 PMID: 5327652
  2. [Individuality of mannonate and altronate hydro-lyases in Escherichia coli K 12].
    Biochimie. 1975;57(1):1-8 PMID: 1096964
  3. Multiple regulation of nucleoside catabolizing enzymes: regulation of the deo operon by the cytR and deoR gene products.
    Mol Gen Genet. 1975;137(4):327-35 PMID: 171553
  4. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  5. Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: pleiotropic constitutive mutations affecting uxu and uidA expression.
    J Bacteriol. 1976 Jul;127(1):418-32 PMID: 776934
  6. Physiological and genetic regulation of the aldohexuronate transport system in Escherichia coli.
    J Bacteriol. 1976 Aug;127(2):706-18 PMID: 783117
  7. Isolation of specialized transducing bacteriophages carrying the structural genes of the hexuronate system in Escherichia coli K-12: exu region.
    J Bacteriol. 1978 Feb;133(2):549-57 PMID: 342497
  8. Regulation of Escherichia coli K-12 hexuronate system genes: exu regulon.
    J Bacteriol. 1980 Sep;143(3):1095-107 PMID: 6997263
  9. [The kinetics of the biosynthesis of beta-galactosidase in Escherichia coli as a function of growth].
    Biochim Biophys Acta. 1952 Dec;9(6):648-60 PMID: 13032175
  10. On the physical state of the intracellularly accumulates substrates of beta-galactoside-permease in Escherichia coli.
    Biochim Biophys Acta. 1958 Sep;29(3):579-87 PMID: 13584361
  11. Genetic regulatory mechanisms in the synthesis of proteins.
    J Mol Biol. 1961 Jun;3:318-56 PMID: 13718526
  12. NON-INDUCIBLE MUTANTS OF THE REGULATOR GENE IN THE "LACTOSE" SYSTEM OF ESCHERICHIA COLI.
    J Mol Biol. 1964 Apr;8:582-92 PMID: 14153528
  13. [Mutations of the structural gene of 2-keto-3-desoxy-6-P-gluconate aldolase in E. coli K 12].
    Mol Gen Genet. 1972;114(4):305-11 PMID: 4552711
  14. Regulation of the gal operon of Escherichia coli by the capR gene.
    J Biol Chem. 1972 May 25;247(10):2973-8 PMID: 4337501
  15. Multiple regulator gene control of the galactose operon in Escherichia coli K-12.
    J Bacteriol. 1972 Jun;110(3):1089-99 PMID: 4555404
  16. [D-altronate: NAD-oxidoreductase in Escherichia coli K12. Purification, properties, and specificity].
    Eur J Biochem. 1972 Mar 15;26(1):50-61 PMID: 4402917
  17. [Colorimetric determinations of aldonate oxidoreductases of Escherichia coli K 12: applications].
    Biochim Biophys Acta. 1972 Nov 10;289(1):19-27 PMID: 4564054
  18. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens.
    J Mol Biol. 1972 Feb 14;63(3):483-503 PMID: 4552408
  19. [Genetic mapping and biochemical characterization of mutations affecting altronic hydrolyase structural gene in Escherichia coli K 12].
    Mol Gen Genet. 1972;118(4):335-50 PMID: 4570160
  20. [Genetic mapping and biochemical characterization of mutations in the mannonic hydrolyase structural gene of Escherichia coli K 12].
    Mol Gen Genet. 1972;118(4):351-62 PMID: 4570161
  21. Transcriptional control of the calactose operon by the capR (lon) and capT genes.
    J Bacteriol. 1973 May;114(2):891-3 PMID: 4574707
  22. [Studies of mutations in the uronic isomerase and altronic oxidoreductase structural genes of Escherichia coli K 12 (author's transl)].
    Mol Gen Genet. 1974;128(4):301-19 PMID: 4594010
  23. [Regulation of hexuronate metabolism in Escherichia coli K12. Kinetics of enzyme induction of the hexuronate system].
    Eur J Biochem. 1974 Mar 15;43(1):1-15 PMID: 4365810
  24. [Mutants of E. coli K 12 unable to grow on methyl-beta-D-glucuronide: map l ocation of uid A. locus of the structural gene of beta-D-glucuronidase].
    Mol Gen Genet. 1973;120(4):319-35 PMID: 4568840
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.145.1.211-220.1981
Published
1981-01-00
Pages
211-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217263
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.145.1.211-220.1981 PMC Full Text View journal details
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