The variation in frequency with which markers are transduced by phage P1 is primarily a result of discrimination during recombination.

Molecular & general genetics : MGG ·Vol. 180 ·No. 3 ·1980-00-00 ·Pages 585-9

Newman BJ, Masters M

Abstract

The efficiency of recovery of P1 transductants is marker dependent and normally varies over a 25-fold range. UV irradiation of either transducing lysates for recipient cells results in a selective stimulation of the transduction of markers which are normally transduced poorly. As a result the range in frequency of transduction is reduced to about 3-fold and resembles the gene frequency distribution expected in the donor cells. We conclude that P1 transducing lysates are likely to contain a random sample of donor DNA but that the recombination system of the recipient cell exhibits a preference for the DNA of some regions over that of others. Damage to DNA presumably overrides this specificity.

MeSH Terms

Coliphages/genetics DNA, Bacterial/genetics,radiation effects Escherichia coli/genetics Gene Frequency Genetic Markers Recombination, Genetic/radiation effects Transduction, Genetic/radiation effects Ultraviolet Rays

Chemicals

DNA, Bacterial Genetic Markers

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Newman B J

Masters M

References (14)

14 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268064

Published

1980-00-00

Pages

585-9

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268064

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The variation in frequency with which markers are transduced by phage P1 is primarily a result of discrimination during recombination.

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