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PMID: 7014469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Hyaluronate capsule prevents attachment of group A streptococci to mouse peritoneal macrophages.

Infection and immunity ·Vol. 31 ·No. 3 ·1981-03-00 ·Pages 985-91

Whitnack E, Bisno AL, Beachey EH

Abstract

The antiphagocytic properties of the streptococcal hyaluronic acid capsule were explored in a system in which binding of the organism to the phagocyte, but not ingestion, could occur. The capsule was found to be highly effective in preventing attachment of two strains of group A streptococci to mouse peritoneal macrophages. Variation in attachment with phase of growth in broth culture (low in early log phase, high in late-log phase and thereafter) could be accounted for by production and subsequent loss of capsule. Hyaluronidase treatment removed the capsule and increased adherence; treatment with proteolytic enzymes removed M protein and decreased resistance to phagocytosis in whole human blood but did not remove the capsule or increase adherence to the mouse peritoneal macrophages.

MeSH Terms
Animals Antigens, Bacterial Ascitic Fluid/cytology Bacterial Outer Membrane Proteins Bacterial Proteins/immunology Carrier Proteins Cell Adhesion/drug effects Hyaluronic Acid/pharmacology Macrophages/physiology Mice Phagocytosis/drug effects Streptococcus pyogenes/immunology
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins streptococcal M protein Hyaluronic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Whitnack E
Bisno A L
Beachey E H
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24 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.31.3.985-991.1981
Published
1981-03-00
Pages
985-91
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC351415
Subset
IM
Grants
NIAID NIH HHS · AI-10085 · United States
NIAID NIH HHS · AI-13550 · United States
External Links
PubMed source DOI: 10.1128/iai.31.3.985-991.1981 PMC Full Text View journal details
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