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PMID: 7015332 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Covalent modification and repressed transcription of a gene in hepatoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 2 ·1981-02-00 ·Pages 834-7

Nakhasi HL, Lynch KR, Dolan KP, Unterman RD, Feigelson P

Abstract

When liver cells undergo malignant transformation, certain genes cease being expressed. We have studied the structure of one such gene, whose protein product we have designated hepatic protein 22 (hp22), which is not expressed in the two Morris hepatomas studied. We have prepared a chimeric clone of pBR322 containing cDNA sequences complementary to mRNA coding for this protein. By using this cloned cDNA, we have examined changes in expression of this gene and changes in the restriction pattern of the DNA isolated from normal liver and these hepatomas. In both hepatomas, studies using the isoschizomeric pair of restriction enzymes Msp I and Hpa II have indicated hypermethylation of a cytosine residue within or proximal to the hp22 gene. Other differences in the restriction pattern between normal liver and hepatoma DNA were also detected with EcoRI and Ava I. Thus, in the nontranscribed form of this gene, the DNA has undergone covalent modification, distinguishing these two hepatomas from each other and from normal liver.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA, Neoplasm/genetics DNA, Recombinant/metabolism Escherichia coli/metabolism Genes Kinetics Liver Neoplasms, Experimental/metabolism Male Nucleic Acid Hybridization Protein Biosynthesis Rats Transcription, Genetic
Chemicals
DNA, Neoplasm DNA, Recombinant
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nakhasi H L
Lynch K R
Dolan K P
Unterman R D
Feigelson P
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.2.834
Published
1981-02-00
Pages
834-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319897
Subset
IM
Grants
NCI NIH HHS · F32 CA-06514 · United States
NIADDK NIH HHS · IT32 AM-07328 · United States
NCI NIH HHS · P01-CA22376 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.2.834 PMC Full Text View journal details
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