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PMID: 7017350 Published · ppublish English Journal Article

Petite-negative mutants of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 181 ·No. 3 ·1981-00-00 ·Pages 409-10

Lancashire WE, Payton MA, Webber MJ, Hartley BS

Abstract

A series of yeast mutants has been isolated with the inability to grow on fermentable carbon sources whilst growing normally on ethanol media. One of the mutants, namely MC16/206 lacks pyruvate decarboxylase activity and does not grow on glucose at 37 degrees C but grows on both ethanol and glucose at 27 degrees C. In this strain rho- petites are non-viable.

MeSH Terms
Alcohol Oxidoreductases/genetics Carboxy-Lyases/genetics DNA, Mitochondrial/genetics Energy Metabolism Genes Mutation Pyruvate Decarboxylase/genetics Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA, Mitochondrial Alcohol Oxidoreductases Carboxy-Lyases Pyruvate Decarboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lancashire W E
Payton M A
Webber M J
Hartley B S
References (6)
6 references, click to expand
  1. Isolation and characterization of Saccharomyces cerevisiae glycolytic pathway mutants.
    J Bacteriol. 1977 May;130(2):746-9 PMID: 400791
  2. Genetic modification of energy-conserving systems in yeast mitochondria.
    Proc Natl Acad Sci U S A. 1968 May;60(1):186-93 PMID: 4232013
  3. Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure.
    Biochim Biophys Acta. 1975 Oct 20;405(2):492-9 PMID: 1101964
  4. A glucokinase from Saccharomyces cerevisiae.
    J Biol Chem. 1970 May 10;245(9):2423-31 PMID: 5442282
  5. Biochemical genetics of oxidative phosphorylation.
    Science. 1967 Dec 22;158(3808):1564-7 PMID: 4228763
  6. A technique for the isolation of yeast alcohol dehydrogenase mutants with altered substrate specificity.
    Arch Biochem Biophys. 1975 Apr;167(2):627-37 PMID: 164834
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1981-00-00
Pages
409-10
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
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