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PMID: 7020949 Published · ppublish English Journal Article

Immunohistochemical localization of cholinergic nerve terminals.

Cell and tissue research ·Vol. 218 ·No. 2 ·1981-00-00 ·Pages 355-73

Jones RT, Walker JH, Richardson PJ, Fox GQ, Whittaker VP

Abstract

Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.

MeSH Terms
Acetylcholinesterase/metabolism Animals Complement Fixation Tests Diaphragm/innervation Electric Organ/innervation Fishes Fluorescent Antibody Technique Ganglia, Sympathetic/ultrastructure Guinea Pigs Hippocampus/ultrastructure Ileum/innervation Parasympathetic Nervous System/ultrastructure Rats Synaptosomes/ultrastructure
Chemicals
Acetylcholinesterase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jones R T
Walker J H
Richardson P J
Fox G Q
Whittaker V P
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32 references, click to expand
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00210350
Published
1981-00-00
Pages
355-73
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00210350 View journal details
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