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PMID: 7022127 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DNA sequence of a plasmid-encoded dihydrofolate reductase.

Molecular & general genetics : MGG ·Vol. 181 ·No. 4 ·1981-00-00 ·Pages 441-7

Swift G, McCarthy BJ, Heffron F

Abstract

The sequence of the methotrexate-resistant dihydrofolate reductase (DHFR) gene borne by the plasmid R-388 was determined. The gene was subcloned and mapped by an in vitro mutagenesis method involving insertion of synthetic oligonucleotide decamers encoding the BamHI recognition site. Sites of insertion that destroyed the methotrexate resistance fell in two regions separated by 300 bp within a 1.2 kb fragment. One of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR. The second region essential for DHFR expression appears to be the promoter of the DHFR gene.

MeSH Terms
Base Sequence Cloning, Molecular Escherichia coli/genetics Genes Genetic Complementation Test Mutation Plasmids Tetrahydrofolate Dehydrogenase/biosynthesis,genetics
Chemicals
Tetrahydrofolate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Swift G
McCarthy B J
Heffron F
References (23)
23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00428733
Published
1981-00-00
Pages
441-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 20287 · United States
Databases
GENBANK
J01773
External Links
PubMed source DOI: 10.1007/BF00428733
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