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PMID: 7022128 Published · ppublish English Journal Article

Acetohydroxy acid synthase isoenzymes of Escherichia coli K-12: a trans-acting regulatory locus of ilvHI gene expression.

Molecular & general genetics : MGG ·Vol. 181 ·No. 4 ·1981-00-00 ·Pages 491-6

Ursini MV, Arcari P, De Felice M

Abstract

We isolated an Escherichia coli K-12 regulatory mutation affecting the acetohydroxy acid synthase III isoenzyme. This mutation was found to lie outside the structural genes ilvHI for this isoenzyme and was designated lrs-1. A strain carrying this mutation was found to be altered in the leucine-mediated control of ilvHI mRNA and acetohydroxy acid synthase III synthesis observed in the isogenic lrs+ strain. These alterations appeared to be a consequence of a reduced intracellular concentration of a single one of five tRNALeu isoaccepting species.

MeSH Terms
Acetolactate Synthase/genetics Escherichia coli/genetics Gene Expression Regulation Genes, Dominant Genes, Regulator Isoenzymes/genetics Isoleucine/biosynthesis Leucine/biosynthesis Mutation Oxo-Acid-Lyases/genetics RNA, Messenger/biosynthesis RNA, Transfer, Amino Acyl/metabolism Valine/biosynthesis
Chemicals
Isoenzymes RNA, Messenger RNA, Transfer, Amino Acyl Isoleucine Acetolactate Synthase Oxo-Acid-Lyases Leucine Valine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ursini M V
Arcari P
De Felice M
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25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00428741
Published
1981-00-00
Pages
491-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00428741
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