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PMID: 7022449 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 6 ·1981-06-00 ·Pages 3450-3

Krasin F, Hutchinson F

Abstract

The repair of DNA double-strand breaks in Escherichia coli cells irradiated with gamma rays occurs only after new proteins are synthesized in response to damage introduced in the genome DNA. One protein whose synthesis is thus induced is the recA protein, and previous work has shown that recA- cells do not repair double-strand breaks. However, inducing recA protein by treating cells with nalidixic acid does not induce repair of double-strand breaks, so this repair requires more than the presence of the recA protein. When repair of double-strand breaks is blocked, the genome DNA is degraded by an endonuclease-like action. Evidence is presented to show that the inducible inhibition of DNA degradation after x-irradiation [Pollard, E. C. & Randall, E. P. (1973) Radiat. Res. 55, 265] is probably caused by the inducible repair of DNA double-strand breaks.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics DNA Repair/drug effects DNA, Bacterial/genetics,radiation effects Escherichia coli/genetics Gamma Rays Rec A Recombinases Recombination, Genetic Rifampin/pharmacology Ultraviolet Rays
Chemicals
Bacterial Proteins DNA, Bacterial Rec A Recombinases Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krasin F
Hutchinson F
References (29)
29 references, click to expand
  1. Repair of DNA double-strand breaks in Escherichia coli, which requires recA function and the presence of a duplicate genome.
    J Mol Biol. 1977 Oct 15;116(1):81-98 PMID: 338918
  2. Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4772-6 PMID: 337300
  3. Induced radioresistance in four strains of Escherichia coli, two with lambda lysogens.
    Biophys J. 1978 Jun;22(3):431-8 PMID: 352423
  4. Escherichia coli recA gene product inactivates phage lambda repressor.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4714-8 PMID: 368796
  5. ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):126-30 PMID: 370822
  6. Degradation of Escherichia coli DNA: evidence for limitation in vivo by protein X, the recA gene product.
    Mol Gen Genet. 1979 Jan 5;168(1):69-80 PMID: 372741
  7. Purified Escherichia coli recA protein catalyzes homologous pairing of superhelical DNA and single-stranded fragments.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1638-42 PMID: 156361
  8. Initiation of general recombination catalyzed in vitro by the recA protein of Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2615-9 PMID: 379861
  9. Single strands induce recA protein to unwind duplex DNA for homologous pairing.
    Nature. 1979 Sep 20;281(5728):191-5 PMID: 225671
  10. Homologous pairing in genetic recombination: complexes of recA protein and DNA.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5100-4 PMID: 159453
  11. Mutagenesis of ultraviolet-irradiated lambda phage by host cell irradiation: induction of Weigle mutagenesis is not an all-or-none process.
    Mol Gen Genet. 1980 Jan;177(2):207-11 PMID: 6445034
  12. Recognition of duplex DNA containing single-stranded regions by recA protein.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2569-73 PMID: 6446716
  13. Homologous pairing in genetic recombination: formation of D loops by combined action of recA protein and a helix-destabilizing protein.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2606-10 PMID: 6994103
  14. DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2819-23 PMID: 6771759
  15. Cleavage of the Escherichia coli lexA protein by the recA protease.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3225-9 PMID: 6447873
  16. DNA synthesis inhibition and the induction of protein X in Escherichia coli.
    J Mol Biol. 1976 Mar 15;101(4):459-77 PMID: 57241
  17. Changes of membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli.
    J Biol Chem. 1970 Nov 10;245(21):5813-9 PMID: 4919490
  18. Role of DNA polymerase I and the rec system in excision-repair in Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1156-60 PMID: 4556455
  19. Studies on the inducible inhibitor of radiation-induced DNA degradation of Escherichia coli.
    Radiat Res. 1973 Aug;55(2):265-79 PMID: 4579016
  20. Proteolytic cleavage of bacteriophage lambda repressor in induction.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):147-51 PMID: 1090931
  21. Inducible error-prone repair in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2753-7 PMID: 1101265
  22. Induction of radioresistance in Escherichia coli.
    Biophys J. 1975 Nov;15(11):1141-54 PMID: 1103984
  23. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  24. Protection of Escherichia coli cells against the lethal effects of ultraviolet and x irradiation by prior x irradiation: a genetic and physiological study.
    Photochem Photobiol. 1976 Dec;24(6):515-23 PMID: 798210
  25. Induction of protein synthesis in Escherichia coli following UV- or gamma-irradiation, mitomycin C treatment or tif Expression.
    Mol Gen Genet. 1977 Feb 28;151(1):57-67 PMID: 325368
  26. Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2283-7 PMID: 329277
  27. Identification of protein X of Escherichia coli as the recA+/tif+ gene product.
    Mol Gen Genet. 1977 Sep 21;155(1):77-85 PMID: 337120
  28. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767-71 PMID: 200930
  29. Identification of the recA (tif) gene product of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5280-4 PMID: 341152
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.6.3450
Published
1981-06-00
Pages
3450-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319586
Subset
IM
Grants
NIGMS NIH HHS · GM 25260 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.6.3450 PMC Full Text View journal details
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