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PMID: 7024909 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Yeast ochre suppressor SUQ5-ol is an altered tRNA Ser UCA.

Nucleic acids research ·Vol. 9 ·No. 13 ·1981-07-10 ·Pages 3077-88

Waldron C, Cox BS, Wills N, Gesteland RF, Piper PW, Colby D, Guthrie C

Abstract

Ochre suppressor tRNA was partially purified from strains of Saccharomyces cerevisiae containing the serine-inserting class III suppressor SUQ5-ol. RNA sequence analysis of this tRNA indicated that the suppressor is derived from a UCA-decoding tRNA Ser by a G leads to U substitution in the middle position of the anticodon. The suppressor further differs from the wild-type UCA-decoding tRNA Ser in that the mutant anticodon lacks the modified uridine found in the wobble position of the wild-type tRNA and contains instead another modification in or near the anticodon.

MeSH Terms
Anticodon/genetics Base Sequence Mutation Nucleic Acid Conformation Oligonucleotides/genetics Oligoribonucleotides/genetics RNA, Transfer/genetics RNA, Transfer, Amino Acyl/genetics Ribonucleases Saccharomyces cerevisiae/genetics Species Specificity Suppression, Genetic
Chemicals
Anticodon Oligonucleotides Oligoribonucleotides RNA, Transfer, Amino Acyl RNA, Transfer Ribonucleases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Waldron C
Cox B S
Wills N
Gesteland R F
Piper P W
Colby D
Guthrie C
References (17)
17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.13.3077
Published
1981-07-10
Pages
3077-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327332
Subset
IM
Grants
NCI NIH HHS · CA13106 · United States
NIGMS NIH HHS · GM21119 · United States
External Links
PubMed source DOI: 10.1093/nar/9.13.3077 PMC Full Text View journal details
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