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PMID: 7024913 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes.

Nucleic acids research ·Vol. 9 ·No. 14 ·1981-07-24 ·Pages 3287-306

Glotz C, Zwieb C, Brimacombe R, Edwards K, Kössel H

Abstract

Short base-paired RNA fragments, and fragments containing intra-RNA cross-links, were isolated from E. coli 23S rRNA or 50S ribosomal subunits by two-dimensional gel electrophoresis. The interactions thus found were used as a first basis for constructing a secondary structure model of the 23S rRNA. Sequence comparison with the 23S rDNA from Z. mays chloroplasts, as well as with the 16S (large subunit) rDNA from human and mouse mitochondria, enabled the experimental model to be improved and extrapolated to give complete secondary structures of all four species. The structures are organized in well-defined domains, with over 450 compensating base changes between the two 23S species. Some ribosomal structural "'switches" were found, one involving 5S rRNA.

MeSH Terms
Animals Base Composition Base Sequence Chloroplasts/analysis Escherichia coli/analysis Humans Mice Mitochondria/analysis Nucleic Acid Conformation Plants/analysis RNA, Ribosomal Ribosomes/analysis Species Specificity Zea mays/analysis
Chemicals
RNA, Ribosomal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Glotz C
Zwieb C
Brimacombe R
Edwards K
Kössel H
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.14.3287
Published
1981-07-24
Pages
3287-306
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327352
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.14.3287 PMC Full Text View journal details
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