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PMID: 7024920 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Determination of base pairing in yeast 5S and 5.8S RNA infrared spectroscopy.

Nucleic acids research ·Vol. 9 ·No. 15 ·1981-08-11 ·Pages 3851-61

Stulz J, Ackermann T, Appel B, Erdmann VA

Abstract

Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are large contributions of tertiary interactions. The results are compared with data derived from structural models that were proposed in the literature as well as with data previously published for prokaryotic 5S RNAs.

MeSH Terms
Base Composition Models, Structural Nucleic Acid Conformation RNA, Fungal Saccharomyces cerevisiae/analysis Spectrophotometry, Infrared Temperature
Chemicals
RNA, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stulz J
Ackermann T
Appel B
Erdmann V A
References (23)
23 references, click to expand
  1. Polynucleotides. VI. Interaction between polyguanylic acid and polycytidylic acid.
    Proc Natl Acad Sci U S A. 1965 Jun;53(6):1425-30 PMID: 5217645
  2. Amount of adenine and uracil base pairs in E. coli 23S, 16S and 5S ribosomal RNA.
    Nature. 1968 Apr 6;218(5136):92-3 PMID: 4868442
  3. Determination of the base pairing content of ribonucleic acids by infrared spectroscopy.
    Biopolymers. 1969;7(3):325-34 PMID: 5771563
  4. Optical properties and base pairing of E. coli 5S RNA.
    Biopolymers. 1972;11(5):1031-9 PMID: 4556238
  5. The extent of base pairing in 5 s RNA. Yeast 5 s RNA.
    J Mol Biol. 1972 Dec 30;72(3):741-9 PMID: 4573846
  6. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.
    J Biol Chem. 1973 Jun 10;248(11):3860-75 PMID: 4575197
  7. Nucleotide sequence of 5 S RNA from Torulopsis utilis.
    FEBS Lett. 1974 Mar 15;40(1):106-9 PMID: 4604831
  8. Oligonucleotide binding to the native and denatured conformers of yeast transfer RNA-3 Lea.
    J Mol Biol. 1974 Nov 5;89(3):495-504 PMID: 4613864
  9. Structure of yeast phenylalanine transfer RNA at 2.5 A resolution.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4414-8 PMID: 1105583
  10. Hydrogen bonding in yeast phenylalanine transfer RNA.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4866-70 PMID: 1108007
  11. Structure and function of 5S and 5.8 S RNA.
    Prog Nucleic Acid Res Mol Biol. 1976;18:45-90 PMID: 790474
  12. Escherichia coli 5S RNA binding proteins L18 and L25 interact with 5.8S RNA but not with 5S RNA from yeast ribosomes.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2706-9 PMID: 142985
  13. Comparative studies on the secondary structure of eukaryotic 5.8S ribosomal RNA.
    Biochemistry. 1977 Aug 23;16(17):3754-9 PMID: 197985
  14. Partial enzyme digestion studies on Escherichia coli, Pseudomonas, Chlorella, Drosophila, HeLa and yeast 5S RNAs support a general class of 5S RNA models.
    J Mol Evol. 1977 Sep 20;10(1):77-86 PMID: 409850
  15. Lasar Raman evidence for a new cloverleaf secondary structure for eucaryotic 5 S RNA.
    J Mol Biol. 1978 Oct 15;125(1):95-105 PMID: 361977
  16. Laser Raman evidence for new cloverleaf secondary structures for eukaryotic 5.8S RNA and prokaryotic 5S RNA.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4901-5 PMID: 368804
  17. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):381-5 PMID: 284354
  18. Determination of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs by infrared spectroscopy.
    Nucleic Acids Res. 1979 Oct 25;7(4):1043-57 PMID: 388350
  19. Physicochemical studies on the 5S ribonucleic acid-protein complex from a eucaryote, Saccharomyces cerevisiae.
    Biochemistry. 1980 Jun 10;19(12):2738-42 PMID: 6994797
  20. A proton-coupled conformational switch of Escherichia coli 5S ribosomal RNA.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3360-4 PMID: 6158045
  21. Melting of Saccharomyces cerevisiae 5S ribonucleic acid: ultraviolet absorption, circular dichroism, and 360-MHz proton nuclear magnetic resonance spectroscopy.
    Biochemistry. 1980 Nov 11;19(23):5456-62 PMID: 7004487
  22. The purine and pyrimidine composition of deoxypentose nucleic acids.
    Biochem J. 1951 May;48(5):584-90 PMID: 14838906
  23. Enzymic synthesis of polynucleotides. I. Polynucleotide phosphorylase of azotobacter vinelandii.
    Biochim Biophys Acta. 1956 Apr;20(1):269-85 PMID: 13315374
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.15.3851
Published
1981-08-11
Pages
3851-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327396
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.15.3851 PMC Full Text View journal details
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