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PMID: 7029471 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Studies on the selectivity of DNA precipitation by spermine.

Nucleic acids research ·Vol. 9 ·No. 20 ·1981-10-24 ·Pages 5493-504

Hoopes BC, McClure WR

Abstract

We have examined the selectivity of the precipitation of DNA by spermine. We have found that the intra- and intermolecular condensation of DNA induced by spermine is highly selective even in the presence of added protein or triphosphates. We have also investigated the influence of buffer components on the threshold concentration of spermine required for DNA precipitation. Representative applications exploiting the selectivity of the precipitation reaction are also described.

MeSH Terms
Animals Bacterial Proteins Cattle Chemical Phenomena Chemical Precipitation Chemistry DNA DNA Polymerase I/metabolism Escherichia coli/enzymology Osmolar Concentration Spermine Thymus Gland
Chemicals
Bacterial Proteins Spermine DNA DNA Polymerase I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hoopes B C
McClure W R
References (11)
11 references, click to expand
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    J Biol Chem. 1978 Dec 25;253(24):8941-8 PMID: 363712
  8. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.
    J Biol Chem. 1973 Jun 10;248(11):3860-75 PMID: 4575197
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    J Mol Biol. 1978 May 25;121(3):311-26 PMID: 671540
  10. Compact form of DNA induced by spermidine.
    Nature. 1976 Jan 29;259(5541):333-5 PMID: 1250371
  11. Mechanism of the antibacterial action of spermine.
    Arch Biochem Biophys. 1959 Mar;81(1):36-54 PMID: 13637964
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.20.5493
Published
1981-10-24
Pages
5493-504
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327535
Subset
IM
Grants
PHS HHS · 21052 · United States
NCRR NIH HHS · RR00995 · United States
External Links
PubMed source DOI: 10.1093/nar/9.20.5493 PMC Full Text View journal details
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