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PMID: 7031033 Published · ppublish English Journal Article

Effect of nalidixic acid and novobiocin on pBR322 genetic expression in Escherichia coli minicells.

Journal of bacteriology ·Vol. 148 ·No. 3 ·1981-12-00 ·Pages 745-52

Gómez-Eichelmann MC

Abstract

The effects of two deoxyribonucleic acid (DNA) gyrase inhibitors, nalidixic acid and novobiocin, on the gene expression of plasmid pBR322 in Escherichia coli minicells were studied. Quantitative estimates of the synthesis of pBR322-coded polypeptides in novobiocin-treated minicells showed that the synthesis of a polypeptide of molecular weight of 34,000 (the tetracycline resistance protein) was reduced to 11 to 20% of control levels, whereas the amount of a polypeptide of 30,500 (the beta-lactamase precursor) was increased to as much as 200%. Nalidixic acid affected the synthesis of the tetracycline resistance protein similarly to novobiocin, although to a lesser extent. The effects of nalidixic acid were not observed in a nalidixic-resistant mutant; those induced by novobiocin were only partially suppressed in a novobiocin-resistant mutant. The synthesis of one of the inducible tetracycline-resistant proteins (34,000) coded by plasmid pSC101 was also reduced in nalidixic acid- and novobiocin-treated minicells. These results suggest that the gyrase inhibitors modified the interaction of ribonucleic acid polymerase with some promoters, either by decreasing the supercoiling density of plasmid DNA or by altering the association constant of the gyrase to specific DNA sites.

MeSH Terms
Bacterial Proteins/metabolism Escherichia coli/drug effects,genetics Gene Expression Regulation/drug effects Half-Life Nalidixic Acid/pharmacology Novobiocin/pharmacology Plasmids Protein Precursors/metabolism Tetracycline/pharmacology beta-Lactamases/metabolism
Chemicals
Bacterial Proteins Protein Precursors Novobiocin Nalidixic Acid beta-Lactamases Tetracycline
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gómez-Eichelmann M C
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36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.148.3.745-752.1981
Published
1981-12-00
Pages
745-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216271
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.148.3.745-752.1981 PMC Full Text View journal details
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