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PMID: 7033751 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Plasmids coding for enterotoxins, K88 antigen and colicins in porcine Escherichia coli strains of O-group 149.

Medical microbiology and immunology ·Vol. 170 ·No. 2 ·1981-00-00 ·Pages 63-72

Franklin A, Söderlind, Möllby R

Abstract

This study was carried out to determine whether the strong epidemiological correlation observed in Sweden between production of the adhesin K88, the heat-stable (ST) and the heat-labile (LT) enterotoxins in E. coli strains of O-group 149 isolated from piglet diarrhea might be explained by linkage of their genetic determinants. From 22 different isolates plasmids coding for these virulence factors were investigated by conjugation and transduction experiments and analysis on agarose gels. The genes coding for ST production could be transferred by selection for antibiotic resistance, but behaved as transposable elements most often residing on a 55 Mdal plasmid coding for colicin B. The genes coding for raffinose fermentation and K88 antigen production were located on a 45 Mdal plasmid and the genes coding for LT production on plasmids within the 45-70 Mdal size. Thus the epidemiological importance and spread of this O-group in Sweden was explained by its stable content of two or three virulence plasmids, which could be transferred independently of one another.

MeSH Terms
Animals Antigens, Bacterial/genetics Diarrhea/immunology,physiopathology,veterinary Enterotoxins/genetics Escherichia coli/genetics Genetic Techniques Plasmids Swine Swine Diseases/immunology,physiopathology
Chemicals
Antigens, Bacterial Enterotoxins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Franklin A
Söderlind
Möllby R
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25 references, click to expand
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Article Info
Journal
Medical microbiology and immunology
Abbr.
Med Microbiol Immunol
ISSN
0300-8584
DOI
10.1007/BF02122670
Published
1981-00-00
Pages
63-72
Language
English
Region
Germany
NLM ID
0314524
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02122670 View journal details
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