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PMID: 7037639 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Surface expression of a nonstructural antigen on influenza A virus-infected cells.

Infection and immunity ·Vol. 34 ·No. 3 ·1981-12-00 ·Pages 1065-7

Shaw MW, Lamon EW, Compans RW

Abstract

Rabbit antiserum to the influenza A virus nonstructural protein, NS1, was used for indirect immunofluorescence studies of infected cells. Nonstructural antigens were detected on surfaces of P815 cells as early as 4 h after infection with A/WSN/40 virus. Adsorption of the serum with virion structural proteins did not affect the observed fluorescence, and a progressive increase in surface fluorescence at later times postinfection indicated that the surface antigen was newly synthesized during the replication cycle.

MeSH Terms
Animals Antigens, Viral/analysis Cell Membrane/immunology Cells, Cultured Fluorescent Antibody Technique Influenza A virus/immunology Mice Molecular Weight Orthomyxoviridae Infections/immunology
Chemicals
Antigens, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shaw M W
Lamon E W
Compans R W
References (18)
18 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.34.3.1065-1067.1981
Published
1981-12-00
Pages
1065-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350976
Subset
IM
Grants
NIAID NIH HHS · AI-12680 · United States
NCI NIH HHS · CA-09202 · United States
NCI NIH HHS · CA-17273 · United States
External Links
PubMed source DOI: 10.1128/iai.34.3.1065-1067.1981 PMC Full Text View journal details
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