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PMID: 7043468 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 6 ·1982-03-00 ·Pages 1935-9

Figurski DH, Pohlman RF, Bechhofer DH, Prince AS, Kelton CA

Abstract

Cloning of specific regions of RK2, a broad host range incompatibility group P plasmid, has revealed three genes: kilA, kilB, and kilC. Each of these genes can cause loss of viability of an Escherichia coli host. This effect on the host is normally prevented by the functions of three additional RK2 genes: korA, korB, and korC. Each kor gene is specific for a particular kil gene. The kil and kor genes are located in four distinct regions of the RK2 genome. The three kil genes are not clustered and, with the possible exception of kilA, they are also well separated from their corresponding kor genes. We have found that the korA and korB determinants are not peculiar to RK2 but instead are highly conserved throughout the incompatibility group P plasmids.

MeSH Terms
Cell Survival Escherichia coli/genetics Genes Genes, Bacterial Phenotype Plasmids
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Figurski D H
Pohlman R F
Bechhofer D H
Prince A S
Kelton C A
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.6.1935
Published
1982-03-00
Pages
1935-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346096
Subset
IM
Grants
NIAID NIH HHS · AI 07161 · United States
NCI NIH HHS · CA 13636 · United States
NIGMS NIH HHS · GM 26863 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.6.1935 PMC Full Text View journal details
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