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PMID: 7048028 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Isolation and mapping of a uracil-sensitive mutant of Salmonella typhimurium.

Molecular & general genetics : MGG ·Vol. 185 ·No. 3 ·1982-00-00 ·Pages 513-4

Bussey LB, Ingraham JL

Abstract

A uracil-sensitive mutant of Salmonella typhimurium was isolated by diethyl sulfate mutagenesis and penicillin counterselection. This mutation identifies a new Salmonella gene that is well separated from the structural genes for arginine and pyrimidine biosynthesis. The use-1 mutation was located between the ilv gene cluster (isoleucine-valine operon) and hisR (structural gene for histidine tRNA) at 83 map units.

MeSH Terms
Crosses, Genetic Genes/drug effects Mutagens/pharmacology Mutation Salmonella typhimurium/drug effects,genetics,isolation & purification Sulfuric Acid Esters/pharmacology Transduction, Genetic Uracil/metabolism
Chemicals
Mutagens Sulfuric Acid Esters Uracil diethyl sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bussey L B
Ingraham J L
References (10)
10 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334151
Published
1982-00-00
Pages
513-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334151
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