Conformational transitions of poly(dA-dC).poly(dG-dT) induced by high salt or in ethanolic solution.

Nucleic acids research ·Vol. 10 ·No. 3 ·1982-02-11 ·Pages 1081-91

Zimmer C, Tymen S, Marck C, Guschlbauer W

Abstract

Poly(dA-dC).poly(dG-dT) was studied by circular dichroism in the presence of high CsCl concentrations and in ethanolic solutions. This alternating purine-pyrimidine duplex may undergo two conformational transitions from a B-type to a novel structure and subsequently into an A-form. Cs+ ions or increasing ethanol concentrations induced a change of the B-type CD spectrum and an inversion of the long wavelength CD band. Lowering the temperature below 0 C or addition of small amounts of Ca++ ions were particularly potent in producing a large negative CD band. A modified B-type structure or a conversion into a left-handed Z-form is considered for this conformational transition.

MeSH Terms

Circular Dichroism Ethanol Nucleic Acid Conformation Oligodeoxyribonucleotides Oligonucleotides Osmolar Concentration Polydeoxyribonucleotides

Chemicals

Oligodeoxyribonucleotides Oligonucleotides Polydeoxyribonucleotides Ethanol poly(dA-dC).poly(dG-dT)

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Zimmer C

Tymen S

Marck C

Guschlbauer W

References (29)

29 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.3.1081

Published

1982-02-11

Pages

1081-91

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326222

Subset

External Links

PubMed source DOI: 10.1093/nar/10.3.1081 PMC Full Text View journal details

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Conformational transitions of poly(dA-dC).poly(dG-dT) induced by high salt or in ethanolic solution.

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