Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction.
Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding.
18 references, click to expand
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Three-dimensional visualization of coated vesicle formation in fibroblasts.J Cell Biol. 1980 Mar;84(3):560-83 PMID: 6987244
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The galactose-specific recognition system of mammalian liver: the route of ligand internalization in rat hepatocytes.Cell. 1980 Aug;21(1):79-93 PMID: 7407914
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