A large particle associated with the perimeter of the nuclear pore complex.

The Journal of cell biology ·Vol. 93 ·No. 1 ·1982-04-00 ·Pages 63-75

Unwin PN, Milligan RA

Abstract

The three-dimensional structure of the nuclear pore complex has been determined to a resolution of approximately 90 A by electron microscopy using nuclear envelopes from Xenopus oocytes. It is shown to be an assembly of several discrete constituents arranged with octagonal symmetry about a central axis. There are apparent twofold axes perpendicular to the octad axis which suggest that the framework of the pore complex is constructed from two equal but oppositely facing halves. The half facing the cytoplasm is in some instances decorated by large particles, similar in appearance and size to ribosomes.

MeSH Terms

Animals Female Membrane Proteins/analysis Microscopy, Electron Nuclear Envelope/ultrastructure Nucleoproteins/analysis Oocytes/ultrastructure Ribosomes/ultrastructure Xenopus

Chemicals

Membrane Proteins Nucleoproteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Unwin P N

Milligan R A

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29 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.93.1.63

Published

1982-04-00

Pages

63-75

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2112107

Subset

External Links

PubMed source DOI: 10.1083/jcb.93.1.63 PMC Full Text View journal details

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A large particle associated with the perimeter of the nuclear pore complex.

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