Home LiteratureArticle Details
PMID: 7110133 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic instability at the adenine phosphoribosyltransferase locus in mouse L cells.

Molecular and cellular biology ·Vol. 2 ·No. 3 ·1982-03-00 ·Pages 250-7

Tischfield JA, Trill JJ, Lee YI, Coy K, Taylor MW

Abstract

Resistance to adenine analogs such as 2,6-diaminopurine occurs at a rate of approximately 10(-3) per cell per generation in mouse L cells. This resistance is associated with a loss of detectable adenine phosphoribosyltransferase activity. Other genetic loci in L cells have the expected mutation frequency (approximately 10(-6)). Transformation of L cell mutants with Chinese hamster ovary cell DNA results in transformants with adenine phosphoribosyltransferase activity characteristic of Chinese hamster ovary cells. No activation of the mouse gene occurs on hybridization with human fibroblasts. That this high frequency event is the result of mutation rather than an epigenetic event is supported by antigenic and reversion studies of the 2,6-diaminopurine-resistant clones. These results are consistent with either a mutational hot-spot, a locus specific mutator gene, or a site of integration of an insertion sequence.

MeSH Terms
Adenine Phosphoribosyltransferase/genetics,immunology,metabolism Animals Cells, Cultured Clone Cells/enzymology Cricetinae Cricetulus Cross Reactions Female Gene Expression Regulation Genes Humans Hybrid Cells/enzymology L Cells/enzymology Mice Mutation Ovary Pentosyltransferases/genetics Transformation, Genetic
Chemicals
Pentosyltransferases Adenine Phosphoribosyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tischfield J A
Trill J J
Lee Y I
Coy K
Taylor M W
References (34)
34 references, click to expand
  1. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  2. The spontaneous azaguanine-resistant mutants of diploid human fibroblasts.
    Humangenetik. 1972;16(1):87-110 PMID: 4647448
  3. Transforming DNA integrates into the host chromosome.
    Cell. 1981 Jan;23(1):29-39 PMID: 7214526
  4. Appearance of hypoxanthine guanine phosphoribosyltransferase activity as a consequence of mycoplasma contamination.
    Nature. 1975 Jul 24;256(5515):329-31 PMID: 1143337
  5. Retroviruses as mutagens: insertion and excision of a nontransforming provirus alter expression of a resident transforming provirus.
    Cell. 1981 Jul;25(1):23-36 PMID: 6268304
  6. Suppression of vesicular stomatitis virus defective intefering particle generation by a function(s) associated with human chromosome 16.
    J Virol. 1981 Dec;40(3):946-52 PMID: 6275129
  7. Gene inactivation as a mechanism for the generation of variability in somatic cells cultivated in vitro.
    Mutat Res. 1977 Sep;44(3):391-400 PMID: 904649
  8. Assignment of the gene for adenine phosphoribosyltransferase to human chromosome 16 by mouse-human somatic cell hybridization.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):45-9 PMID: 4129802
  9. Purification of adenine phosphoribosyltransferase by affinity chromatography.
    Prep Biochem. 1978;8(6):453-62 PMID: 748918
  10. Reversible inactivation of autosomal alleles in Chinese hamster cells.
    J Cell Physiol. 1979 Nov;101(2):325-40 PMID: 511955
  11. Mutator gene studies in Escherichia coli: the mutT gene.
    Genetics. 1973 Apr;73:Suppl 73:67-80 PMID: 4576120
  12. Genetic and biochemical studies with the adenosine analogs toyocamycin and tubercidin: mutation at the adenosine kinase locus in Chinese hamster cells.
    Somatic Cell Genet. 1978 Nov;4(6):715-35 PMID: 217113
  13. Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNA.
    Somatic Cell Genet. 1980 May;6(3):333-47 PMID: 6931407
  14. Transposable elements.
    Cell. 1980 Jul;20(3):579-95 PMID: 6251969
  15. Development of a nonselective technique for studying 2, 6, diaminopurine resistance in an established murine cell line.
    Genetics. 1968 Dec;60(4):781-92 PMID: 5753549
  16. A table for the estimation of the spontaneous mutation rate of cells in culture.
    Mutat Res. 1973 Jan;17(1):147-8 PMID: 4682600
  17. The origin and behavior of mutable loci in maize.
    Proc Natl Acad Sci U S A. 1950 Jun;36(6):344-55 PMID: 15430309
  18. A Factor (or Mutator Gene) Influencing Mutation Rates in Escherichia Coli.
    Proc Natl Acad Sci U S A. 1954 Nov;40(11):1064-71 PMID: 16578437
  19. High frequency of mutation to tubercidin resistance in CHO cells.
    Somatic Cell Genet. 1979 Sep;5(5):571-83 PMID: 294700
  20. Altered enzymes in drug-resistant variants of mammalian tissue culture cells.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3145-9 PMID: 4522295
  21. Mutants of cultured chinese hamster cells deficient in adenine phosphoribosyl transferase.
    Cell. 1974 May;2(1):43-54 PMID: 4472208
  22. Gene linkage analysis in the mouse by somatic cell hybridization: assignment of adenine phosphoribosyltransferase to chromosome 8 and alpha-galactosidase to the X chromosome.
    Somatic Cell Genet. 1975 Oct;1(4):371-82 PMID: 1235912
  23. Mutations of Bacteria from Virus Sensitivity to Virus Resistance.
    Genetics. 1943 Nov;28(6):491-511 PMID: 17247100
  24. The relationship between rates of ( 3 H)uridine and ( 3 H)adenine incorporation into RNA and the measured rates of RNA synthesis during the cell cycle.
    Biochim Biophys Acta. 1972 Sep 29;281(1):45-54 PMID: 4673465
  25. On the nature of hereditable variation in cultured somatic cells.
    Cell. 1976 Jan;7(1):1-11 PMID: 779953
  26. Dimethyl sulfoxide enhances polyethylene glycol-mediated somatic cell fusion.
    Somatic Cell Genet. 1976 May;2(3):263-70 PMID: 1028173
  27. Origin of retroviruses from cellular moveable genetic elements.
    Cell. 1980 Oct;21(3):599-600 PMID: 6254661
  28. A new reduced human-mouse somatic cell hybrid containing the human gene for adenine phosphoribosyltransferase.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):82-6 PMID: 5276304
  29. Diaminopurine-resistant mutants of cultured, diploid human fibroblasts.
    Genetics. 1973 Oct;75(2):335-45 PMID: 4358687
  30. A new electrophoretic-autoradiographic method for the visual detection of phosphotransferases.
    Anal Biochem. 1973 Jun;53(2):545-54 PMID: 4351949
  31. Purine mutants of mammalian cell lines: III. Control of purine biosynthesis in adenine phosphoribosyl transferase mutants of CHO cells.
    Somatic Cell Genet. 1977 Mar;3(2):195-206 PMID: 75575
  32. Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detection.
    Mutat Res. 1974 Sep;24(3):335-64 PMID: 4607023
  33. Mouse teratocarcinoma mutant clones deficient in adenine phosphoribosyltransferase and developmentally pluripotent.
    Somatic Cell Genet. 1979 Nov;5(6):781-92 PMID: 296585
  34. Tissue cultures and mycoplasmas.
    Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+ PMID: 5833547
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.3.250-257.1982
Published
1982-03-00
Pages
250-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369783
Subset
IM
Grants
NIADDK NIH HHS · AM25498 · United States
NIGMS NIH HHS · GM18924 · United States
NIGMS NIH HHS · GM26917 · United States
External Links
PubMed source DOI: 10.1128/mcb.2.3.250-257.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2