Rapid method for identification and enumeration of oral Actinomyces.

Infection and immunity ·Vol. 21 ·No. 3 ·1978-09-00 ·Pages 786-91

Marucha PT, Keyes PH, Wittenberger CL, London J

Abstract

Serotype-specific antisera prepared against whole cells of Actinomyces viscosus, A. naeslundii, and A. israeli were labeled with fluorescein dye and used to detect and quantitate antigenically related microorganisms in human dental plaque. By relating the DNA content of the dental plaque microflora to the number of Actinomyces present in the plaque samples, a reproducible method was developed for specifically enumerating five serotypic representatives of this genus found in human plaque.

MeSH Terms

Actinomyces/classification,immunology,isolation & purification Antibody Specificity Dental Plaque/microbiology Humans Serotyping

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Marucha P T

Keyes P H

Wittenberger C L

London J

References (13)

13 references, click to expand

SEROLOGICAL GROUPING OF ACTINOMYCES BY MEANS OF FLUORESCENT ANTIBODIES.
J Bacteriol. 1961 Jul;82(1):54-65 PMID: 16561915
Improvement in specificity of immunofluorescent reagents for identifying Streptococcus mutans by DEAE-cellulose-bacterial cell column immunosorption methods.
J Dent Res. 1976 Jan;55:A50-7 PMID: 54376
Conjugation methods in immunofluorescence.
J Dent Res. 1976 Jan;55:A38-44 PMID: 54374
Histological changes in experimental periodontal disease in gnotobiotic rats and conventional hamsters.
J Periodontal Res. 1974;9(2):73-80 PMID: 4277507
Periodontal lesions in hamsters and gnotobiotic rats infected with actinomyces of human origin.
J Periodontal Res. 1972;7(1):21-8 PMID: 4272027
A simple fluorometric method to determine deoxyribonucleic acid in dental plaque.
J Dent Res. 1974 Jul-Aug;53(4):934 PMID: 4526380
Reactions in the periodontium to continuous antigenic stimulation in sensitized gnotobiotic rats.
Infect Immun. 1974 Sep;10(3):565-77 PMID: 4426700
Two new serological groups of Actinomyces.
J Bacteriol. 1970 Jul;103(1):265-6 PMID: 4912526
Rodent model systems in periodontal disease research.
J Dent Res. 1971 Mar-Apr;50(2):236-42 PMID: 4929694
Effect of sonic treatment on pure cultures and aggregates of bacteria.
J Clin Microbiol. 1976 May;3(5):474-9 PMID: 819454
Serological identification of Actinomyces using fluorescent antibody techniques.
J Dent Res. 1976 Jan;55:A184-91 PMID: 1107378
Induction of periodontal destruction in gnotobiotic rats by a human oral strain of Actinomyces naeslundii.
Arch Oral Biol. 1970 Oct;15(10):993-5 PMID: 5275754
Revision of serological grouping of Actinomyces.
J Bacteriol. 1966 May;91(5):2107 PMID: 5937255

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.21.3.786-791.1978

Published

1978-09-00

Pages

786-91

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422066

Subset

External Links

PubMed source DOI: 10.1128/iai.21.3.786-791.1978 PMC Full Text View journal details

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Rapid method for identification and enumeration of oral Actinomyces.

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