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PMID: 71287 Published · ppublish English Journal Article

Osmium tetroxide/p-phenylenediamine staining of nucleoli and Balbiani Rings in Chironomus salivary glands.

Histochemistry ·Vol. 53 ·No. 1 ·1977-07-18 ·Pages 43-56

Stockert JC

Abstract

This paper deals with the application of the osmium tetroxide fixation followed by p-phenylenediamine treatment to salivary gland cells from Chironomus larvae. After this procedure, cytoplasm, nucleoli and Balbiani rings show a high degree of staining both in light and electron microscopy, while chromatin remains unstained. Ethanol fixation followed by osmium tetroxide/p-phenylenediamine does not modify the above mentioned staining pattern. Under these conditions, extractive procedures for lipids do not affect the osmiophilia of nucleoli and Balbiani rings, while RNase or trichloroacetic acid treatment decreaes the staining degree of these structures. In osmium tetroxide/p-phenylenediamine treated salivary glands, the highest contrast within nuclei is seen to occur in the pars granulosa from normal or segregated nucleoli, as well as in Balbiani ring granules, which appear either as hollow granules or with a bipartite or horseshoe-like structure.

MeSH Terms
Animals Cell Nucleolus/drug effects,ultrastructure Chromosomes/drug effects,ultrastructure Cytoplasm/ultrastructure Diptera/ultrastructure Microscopy Microscopy, Electron Osmium Phenylenediamines Ribonucleases/pharmacology Salivary Glands/ultrastructure Staining and Labeling Trichloroacetic Acid/pharmacology
Chemicals
Phenylenediamines Osmium Trichloroacetic Acid Ribonucleases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Stockert J C
References (28)
28 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00511209
Published
1977-07-18
Pages
43-56
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00511209
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