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PMID: 7130277 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and initial characterization of the mammalian midbody.

The Journal of cell biology ·Vol. 94 ·No. 3 ·1982-09-00 ·Pages 654-61

Mullins JM, McIntosh JR

Abstract

Midbodies were isolated from synchronized cultures of Chinese hamster ovary (CHO) cells and their protein composition was studied by means of SDS PAGE. Gels of the midbodies included alpha and beta tubulins as major bands (approximately 30% of the total protein) and approximately 35 other bands, none of which constituted greater than 3.5% of the total protein. Extraction of the isolated midbodies with Sarkosyl NL-30- solubilized the midbody microtubules but left the central, dense matrix zone of the midbody intact. A protein doublet of approximately 115,000 mol wt was retained preferentially by the particulate fraction containing the matrix zones, indicating it to be a component of the matrix. The 115,000 mol wt doublet was also present in gels of isolated mitotic spindles from CHO cells. The overall protein composition of the isolated spindles was very similar to that of the isolated midbodies.

MeSH Terms
Animals Cell Line Cricetinae Cricetulus Intercellular Junctions/ultrastructure Microscopy, Electron Microtubules/ultrastructure Mitosis Molecular Weight Proteins/analysis
Chemicals
Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mullins J M
McIntosh J R
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.94.3.654
Published
1982-09-00
Pages
654-61
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112229
Subset
IM
Grants
NIGMS NIH HHS · GM26802 · United States
External Links
PubMed source DOI: 10.1083/jcb.94.3.654 PMC Full Text View journal details
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