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PMID: 7139029 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Improved fluorescent probes for the measurement of rapid changes in membrane potential.

Biophysical journal ·Vol. 39 ·No. 3 ·1982-09-00 ·Pages 301-8

Grinvald A, Hildesheim R, Farber IC, Anglister L

Abstract

To improve the quality of fluorescent voltage-sensitive probes twenty new styryl dyes were synthesized. Some of the new probes are significantly better than any used in the past. A signal-to-noise ratio of 90 root mean square (rms) noise was obtained for an optical recording of action potentials from neuroblastoma cells maintained in monolayer culture. The fluorescence fractional change of the optical signal is as large as 14%/100 mV. Photodynamic damage and bleaching are much less significant with the new probes. These fluorescent probes can be used to measure small and rapid changes in membrane potential from single cells maintained in monolayer cultures, from single cells in invertebrate ganglia, from their arborization, and from other preparations. The optical measurement can be made with a standard fluorescent microscope equipped with DC mercury illumination. Guidelines for the design of even better fluorescent probes and more efficient instruments are suggested.

MeSH Terms
Animals Cell Line Clone Cells Fluorescent Dyes Ganglia/physiology Leeches Membrane Potentials Mice Neuroblastoma/physiopathology Spectrometry, Fluorescence
Chemicals
Fluorescent Dyes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grinvald A
Hildesheim R
Farber I C
Anglister L
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21 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(82)84520-7
Published
1982-09-00
Pages
301-8
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1328947
Subset
IM
Grants
NINDS NIH HHS · NS 14716 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(82)84520-7 PMC Full Text View journal details
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