Circular molecules of heterogeneous size from mitochondrial fractions of the petite-negative yeast Schizosaccharomyces pombe.

Molecular & general genetics : MGG ·Vol. 164 ·No. 3 ·1978-09-08 ·Pages 289-93

Del Giudice L, Wolf K, Sassone-Corsi P, Alvino C

Abstract

Size and shape of mitochondrial DNA molecules of Schizosaccharomyces pombe were analyzed by electron microscopy. Besides numerous linear molecules, circular molecules ranging from 0.83 micron to 12.81 micron were found. Depending on the method of preparation, both closed and open circular molecules were found. Most of the circular molecules could be assigned to five major size classes of 0.83 +/- 0.05 micron, 1.7 +/- 0.05 micron, 4.74 +/- 0.04 micron, 5.74 +/- 0.04 micron, and 8.32 +/- 0.07 micron. Possible explanations for the different size classes of mitochondrial DNA molecules are discussed.

MeSH Terms

Ascomycota/genetics DNA, Circular DNA, Mitochondrial/isolation & purification Microscopy, Electron Mutation Nucleic Acid Conformation Phenotype Schizosaccharomyces/genetics

Chemicals

DNA, Circular DNA, Mitochondrial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Del Giudice L

Wolf K

Sassone-Corsi P

Alvino C

References (19)

19 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00333159

Published

1978-09-08

Pages

289-93

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00333159

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Circular molecules of heterogeneous size from mitochondrial fractions of the petite-negative yeast Schizosaccharomyces pombe.

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