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PMID: 7143258 Published · ppublish English Journal Article

Calcium- and length-dependent force production in rat ventricular muscle.

The Journal of physiology ·Vol. 329 ·1982-08-00 ·Pages 527-40

Hibberd MG, Jewell BR

Abstract

1. Trabeculae from the right ventricles of rat hearts were ;skinned' by immersion for 30 min in a solution containing the non-ionic detergent Brij-58 at a concentration of 1%.2. The average sarcomere length in the central region of the relaxed preparation was estimated by laser diffraction and set at pre-determined values within the range of 1.9-2.4 mum by adjustment of muscle length. Isometric contractions were then induced by raising the Ca(2+) concentration under carefully controlled chemical conditions.3. The dependence of Ca(2+)-activated force production on sarcomere length over the ascending limb of the length-force relation was examined at Ca(2+) concentrations giving partial and full activation of the contractile system of the muscle.4. The dependence of Ca(2+)-activated force on Ca(2+) concentration was compared at sarcomere lengths on the ascending limb and plateau of the length-force relation.5. The results obtained from both kinds of experiment showed that the sensitivity of the contractile system to Ca(2+) increases with sarcomere length over the ascending limb of the length-force relation.6. Possible explanations for this observation have been discussed.

MeSH Terms
Animals Biometry Calcium/pharmacology Dose-Response Relationship, Drug In Vitro Techniques Myocardial Contraction/drug effects Myofibrils/anatomy & histology Rats Rats, Inbred Strains Stimulation, Chemical Stress, Mechanical
Chemicals
Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hibberd M G
Jewell B R
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33 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1982.sp014317
Published
1982-08-00
Pages
527-40
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1224794
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1982.sp014317 PMC Full Text View journal details
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