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PMID: 7153345 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of two methods for enumeration of mycoplasmas.

Journal of clinical microbiology ·Vol. 16 ·No. 5 ·1982-11-00 ·Pages 959-61

Stemke GW, Robertson JA

Abstract

Two methods of viable cell counts were evaluated for Mycoplasma hominis and Ureaplasma urealyticum: color change unit50 and colony-forming unit. The color change unit50 method gave higher estimates of cell numbers; furthermore, the color change unit50 values correlated better with the DNA content of the cell pellet and the published genome sizes.

MeSH Terms
Cell Count/methods Culture Media Mycoplasma/cytology,growth & development
Chemicals
Culture Media
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stemke G W
Robertson J A
References (11)
11 references, click to expand
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    J Bacteriol. 1971 May;106(2):608-14 PMID: 4929869
  5. The interaction between Mycoplasma hominis and poliovirus in cell culture.
    J Gen Virol. 1973 Sep;20(3):303-10 PMID: 4355327
  6. Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA.
    Anal Biochem. 1974 Feb;57(2):369-76 PMID: 4819731
  7. Inhibition of growth of T-strain mycoplasmas by hydroxamic acids and by aurothiomalate.
    Antimicrob Agents Chemother. 1972 Nov;2(5):340-3 PMID: 4207956
  8. Some effects of growth medium composition on the antigenicity of a T-strain mycoplasma.
    Infect Immun. 1975 Mar;11(3):530-9 PMID: 803928
  9. Mycoplasma hominis: growth, reproduction, and isolation of small viable cells.
    J Bacteriol. 1975 Nov;124(2):1007-18 PMID: 1102522
  10. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma).
    J Clin Microbiol. 1978 Feb;7(2):127-32 PMID: 632344
  11. The mycoplasmas.
    Microbiol Rev. 1978 Jun;42(2):414-70 PMID: 353482
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.16.5.959-961.1982
Published
1982-11-00
Pages
959-61
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC272510
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.16.5.959-961.1982 PMC Full Text View journal details
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