Home LiteratureArticle Details

PMID: 7155900 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular structure of (m5 dC-dG)3: the role of the methyl group on 5-methyl cytosine in stabilizing Z-DNA.

Nucleic acids research ·Vol. 10 ·No. 23 ·1982-12-11 ·Pages 7879-92

Fujii S, Wang AH, van der Marel G, van Boom JH, Rich A

Abstract

The hexamer (m5 dC-dG)3 has been synthesized and its three-dimensional structure determined by a single crystal X-ray diffraction analysis. The structure has been refined to a final R value of 15.6% at 1.3 A resolution. The molecule forms a left-handed Z-DNA helix which is similar to the unmethylated Z-DNA structure. The presence of the methyl group has resulted in slight changes in the twist angle between successive base pairs and modification of some of the interatomic contacts. Methylation of cytosine in the C5 position is associated with a relative destabilization of the B-DNA structure and a stabilization through hydrophobic bonding of the Z-DNA structure.

MeSH Terms

5-Methylcytosine Base Sequence Cytosine/analogs & derivatives,analysis DNA Models, Molecular Nucleic Acid Conformation Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis X-Ray Diffraction

Chemicals

Oligodeoxyribonucleotides Oligonucleotides 5-Methylcytosine tri-(deoxyguanylic acid-deoxycytidylic acid) Cytosine DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Fujii S

Wang A H

van der Marel G

van Boom J H

Rich A

References (12)

12 references, click to expand

Salt-induced co-operative conformational change of a synthetic DNA: equilibrium and kinetic studies with poly (dG-dC).
J Mol Biol. 1972 Jun 28;67(3):375-96 PMID: 5045303
Conformational analysis of the sugar ring in nucleosides and nucleotides. A new description using the concept of pseudorotation.
J Am Chem Soc. 1972 Nov 15;94(23):8205-12 PMID: 5079964
Molecular structure of a left-handed double helical DNA fragment at atomic resolution.
Nature. 1979 Dec 13;282(5740):680-6 PMID: 514347
Left-handed DNA helices.
Nature. 1980 Feb 21;283(5749):743-5 PMID: 7354864
DNA methylation and gene function.
Science. 1980 Nov 7;210(4470):604-10 PMID: 6254144
Left-handed double helical DNA: variations in the backbone conformation.
Science. 1981 Jan 9;211(4478):171-6 PMID: 7444458
Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1619-23 PMID: 6262820
5-Methylcytosine in eukaryotic DNA.
Science. 1981 Jun 19;212(4501):1350-7 PMID: 6262918
The high salt form of poly(dG-dC).poly(dG-dC) is left-handed Z-DNA: Raman spectra of crystals and solutions.
Nucleic Acids Res. 1981 Oct 24;9(20):5443-57 PMID: 7301594
Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes.
Nature. 1981 Dec 3;294(5840):417-22 PMID: 6796893
Conformation and dynamics in a Z-DNA tetramer.
J Mol Biol. 1981 Nov 15;152(4):723-36 PMID: 7334519
Monoclonal antibodies recognize different parts of Z-DNA.
J Biol Chem. 1982 Oct 25;257(20):12081-5 PMID: 7118931

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.23.7879

Published

1982-12-11

Pages

7879-92

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327053

Subset

External Links

PubMed source DOI: 10.1093/nar/10.23.7879 PMC Full Text View journal details

Analysis Services

Molecular structure of (m5 dC-dG)3: the role of the methyl group on 5-methyl cytosine in stabilizing Z-DNA.

Contact

WeChat Official Account