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PMID: 71731 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Crystallographic studies of bovine beta2-microglobulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 8 ·1977-08-00 ·Pages 3345-9

Becker JW, Ziffer JA, Edelman GM, Cunningham BA

Abstract

Crystals of the bovine milk protein lactollin yield x-ray diffraction data extending to a resolution of 2.8 A. Lactollin is a bovine analogue of beta2-microglobulin, a protein that is homologous in amino acid sequence to the constant domains of immunoglobulins and is the light chain of the human and murine major histocompatability antigens. The protein crystallizes in the orthorhombic space group P2(1)2(1)2(1) with a = 77.4, b = 47.9, and c = 34.3 A. The unit cell parameters and physical chemical solution studies indicate that the molecule exists in the crystal and in solution as a single polypeptide chain of 12,000 daltons.

MeSH Terms
Amino Acid Sequence Animals Beta-Globulins Cattle Colostrum Female Humans Immunoglobulin G Immunoglobulin gamma-Chains Milk Milk Proteins Molecular Weight Peptide Fragments/analysis Pregnancy Protein Conformation Trypsin X-Ray Diffraction beta 2-Microglobulin
Chemicals
Beta-Globulins Immunoglobulin G Immunoglobulin gamma-Chains Milk Proteins Peptide Fragments beta 2-Microglobulin Trypsin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Becker J W
Ziffer J A
Edelman G M
Cunningham B A
References (36)
36 references, click to expand
  1. The molecular structure of a dimer composed of the variable portions of the Bence-Jones protein REI refined at 2.0-A resolution.
    Biochemistry. 1975 Nov 4;14(22):4943-52 PMID: 1182131
  2. Comparisons of Bence-Jones proteins and L polypeptide chains of myeloma globulins after hydrolysis with trypsin.
    J Exp Med. 1963 Jul;118:41-53 PMID: 13987374
  3. ISOLATION, CHARACTERIZATION, AND AMINO ACID COMPOSITION OF A NEW CRYSTALLINE PROTEIN, LACTOLLIN, FROM MILK.
    Biochemistry. 1963 Jul-Aug;2:814-7 PMID: 14075119
  4. EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.
    Biochemistry. 1964 Mar;3:297-317 PMID: 14155091
  5. Synthesis of 2 -microglobulin by stimulated lymphocytes.
    J Immunol. 1972 Aug;109(2):407-9 PMID: 4114828
  6. The relationship between 2 -microglobulin and immunoglobulin in cultured human lymphoid cell lines.
    J Exp Med. 1973 Mar 1;137(3):838-43 PMID: 4120289
  7. The small subunit of HL-A antigens is beta 2-microglobulin.
    J Exp Med. 1973 Dec 1;138(6):1608-12 PMID: 4128442
  8. Highly purified papain-solubilized HL-A antigens contain beta2-microglobulin.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):35-9 PMID: 4129801
  9. Beta 2-microglobulin production in vitro by human hematopoietic, mesenchymal, and epithelial cells.
    J Immunol. 1974 Jan;112(1):137-44 PMID: 4130145
  10. Detergent-solubilised H-2 alloantigen is associated with a small molecular weight polypeptide.
    Nature. 1974 Jun 21;249(459):764-5 PMID: 4134396
  11. Subfragments from the Fc fragment of human immunoglobulin G. Isolation and physicochemical charaterization.
    Biochem J. 1968 Mar;107(2):171-8 PMID: 4171016
  12. Isolation and properties of a low molecular weight beta-2-globulin occurring in human biological fluids.
    J Biol Chem. 1968 Aug 10;243(15):4095-103 PMID: 4175239
  13. The three-dimensional structure of the fab' fragment of a human myeloma immunoglobulin at 2.0-angstrom resolution.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3440-4 PMID: 4215080
  14. Dog homologue of human 2 -microglobulin.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2914-7 PMID: 4507614
  15. Multiple common properties of human beta2-microglobulin and the common portion fragment derived from HL-A antigen molecules.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2863-5 PMID: 4517941
  16. The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4298-302 PMID: 4530984
  17. 2 -Microglobulin--a free immunoglobulin domain.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1697-701 PMID: 4558655
  18. The complete amino acid sequence of beta 2-microglobulin.
    Biochemistry. 1973 Nov 20;12(24):4811-22 PMID: 4586824
  19. Association of a beta2-microglobulin-like subunit with H-2 and TL alloantigens on murine thymocytes.
    J Immunol. 1975 Jan;114(1 Pt 1):252-4 PMID: 46243
  20. Thymus leukaemia antigen contains beta2-microglobulin.
    Nature. 1975 Feb 27;253(5494):735-7 PMID: 46589
  21. Partial amino acid sequence of rabbit beta2-microglobulin.
    Science. 1975 Mar 21;187(4181):1079-80 PMID: 46618
  22. Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets.
    Anal Biochem. 1973 Jun;53(2):624-8 PMID: 4716391
  23. Structure of a lambda-type Bence-Jones protein at 3.5-A resolution.
    Biochemistry. 1973 Nov 6;12(23):4620-31 PMID: 4773847
  24. Initiation of protein synthesis at an unusual position in an immunoglobulin gene?
    Science. 1972 Jan 14;175(4018):187-9 PMID: 5008437
  25. The covalent structure of an entire gammaG immunoglobulin molecule.
    Proc Natl Acad Sci U S A. 1969 May;63(1):78-85 PMID: 5257969
  26. The covalent structure of a human gamma G-immunoglobulin. XI. Functional implications.
    Biochemistry. 1970 Aug 4;9(16):3197-205 PMID: 5489773
  27. The covalent structure of a human gamma G-immunoglobulin. II. Isolation and characterization of the cyanogen bromide fragments.
    Biochemistry. 1968 May;7(5):1959-66 PMID: 5650387
  28. Micro diffusion cells for the growth of single protein crystals by means of equilibrium dialysis.
    Arch Biochem Biophys. 1968 Aug;126(2):564-73 PMID: 5672516
  29. Solvent content of protein crystals.
    J Mol Biol. 1968 Apr 28;33(2):491-7 PMID: 5700707
  30. Structure and significance of beta2-microglobulin.
    Fed Proc. 1976 Apr;35(5):1171-6 PMID: 57070
  31. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  32. Partial amino acid sequence of mouse beta2-microglobulin.
    Biochem Biophys Res Commun. 1976 May 17;70(2):425-30 PMID: 59595
  33. Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines.
    Biochim Biophys Acta. 1966 Dec 28;130(2):538-40 PMID: 5972864
  34. Variability of three-dimensional structure in immunoglobulins.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):819-23 PMID: 805424
  35. Three-dimensional structure of immunoglobulins.
    Annu Rev Biochem. 1975;44:639-67 PMID: 806253
  36. Crystallographic structural studies of a human Fc-fragment. I. An electron-density map at 4 A resolution and a partial model.
    Hoppe Seylers Z Physiol Chem. 1976 Mar;357(3):435-45 PMID: 955567
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.8.3345
Published
1977-08-00
Pages
3345-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431555
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.8.3345 PMC Full Text View journal details
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