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PMID: 7188935 Published · ppublish English Journal Article

Ultrastructural localization of soybean agglutinin on thin sections of Glycine max (soybean) var. Altona by the gold method.

Histochemistry ·Vol. 65 ·No. 2 ·1980-02-00 ·Pages 181-6

Horisberger M, Vonlanthen M

Abstract

Soybean agglutinin (SBA) has been localized in Glycine max (soybean) var. Altona at the ultrastructural level by the gold method. SBA was detected by marking thin sections of different part of the seed with gold granules (12 nm in size) labelled with anti-SBA antiserum. Upon examination by transmission electron microscopy, the lectin was found uniformly distributed in most of the protein bodies of the cotyledon. SBA was also present in the embryo axis.

MeSH Terms
Gold Histocytochemistry Immunoassay/methods Lectins/analysis Microscopy, Electron Plant Lectins Plants/analysis Soybeans/analysis
Chemicals
Lectins Plant Lectins Gold
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Horisberger M
Vonlanthen M
References (15)
15 references, click to expand
  1. Subcellular localization of cellulases in auxin-treated pea.
    J Cell Biol. 1976 Apr;69(1):97-105 PMID: 767348
  2. Role of Lectins in Plant-Microorganism Interactions: II. Distribution of Soybean Lectin in Tissues of Glycine max (L.) Merr.
    Plant Physiol. 1978 May;61(5):779-84 PMID: 16660384
  3. Localization of lectins in legume cotyledons.
    J Cell Sci. 1975 Oct;19(1):157-67 PMID: 1176546
  4. A low-viscosity epoxy resin embedding medium for electron microscopy.
    J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
  5. Ultrastructural localization of beta-D-galactan in the nuclei of the myxomycete Physarum polycephalum.
    Biochim Biophys Acta. 1978 Aug 17;542(2):308-14 PMID: 356891
  6. The lectins: carbohydrate-binding proteins of plants and animals.
    Adv Carbohydr Chem Biochem. 1978;35:127-340 PMID: 356549
  7. Evidence for the presence of proteinase inhibitor I in vacuolar protein bodies of plant cells.
    Planta. 1976 Jan;129(2):161-5 PMID: 24430908
  8. Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections.
    Histochemistry. 1979 Apr 12;60(3):317-20 PMID: 224008
  9. Simultaneous localization of an hepatic binding protein specific for galactose and of galactose-containing receptors on rat hepatocytes.
    J Histochem Cytochem. 1978 Nov;26(11):960-6 PMID: 722052
  10. Location of mannan and chitin on thin sections of budding yeasts with gold markers.
    Arch Microbiol. 1977 Oct 24;115(1):1-7 PMID: 337918
  11. Localization of vicilin peptidohydrolase in the cotyledons of mung bean seedlings by immunofluorescence microscopy.
    J Cell Biol. 1978 Oct;79(1):10-9 PMID: 359572
  12. Colloidal gold, a useful marker for transmission and scanning electron microscopy.
    J Histochem Cytochem. 1977 Apr;25(4):295-305 PMID: 323352
  13. Galactose oxidase. An enzyme with lectin properties.
    Biochim Biophys Acta. 1979 Apr 25;577(2):383-8 PMID: 454653
  14. Localization of alpha-galactomannan and of wheat germ agglutinin receptors in Schizosaccharomyces pombe.
    Arch Microbiol. 1978 Nov 13;119(2):107-11 PMID: 727851
  15. Rapid release of protease inhibitors from soybeans: immunochemical quantitation and parallels with lectins.
    Plant Physiol. 1978 Jan;61(1):30-4 PMID: 16660231
Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
Published
1980-02-00
Pages
181-6
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
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