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PMID: 7190739 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Formation of hybridoma clones in soft agarose: effect of pH and of medium.

Somatic cell genetics ·Vol. 6 ·No. 3 ·1980-05-00 ·Pages 435-41

Sharon J, Morrison SL, Kabat EA

Abstract

Optimal conditions for the formation of hybridoma clones in soft agarose are described. The hybridization frequency is shown to be highly dependent on the pH of the polyethylene glycol (PEG) solution used for fusion and on the cloning medium. Maximal numbers of clones are obtained when the PEG solution used for fusion is at pH 8.0-8.2.

MeSH Terms

Animals Antibody-Producing Cells/cytology Cell Fusion/drug effects Clone Cells/cytology Culture Media Hybrid Cells/cytology Hydrogen-Ion Concentration Mice Myeloma Proteins Neoplasms, Experimental/pathology Plasmacytoma/pathology Polyethylene Glycols/pharmacology Sepharose

Chemicals

Culture Media Myeloma Proteins Polyethylene Glycols Sepharose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sharon J

Morrison S L

Kabat E A

Article Info

Journal

Somatic cell genetics

Abbr.

Somatic Cell Genet

ISSN

0098-0366

DOI

10.1007/BF01542794

Published

1980-05-00

Pages

435-41

Language

English

Region

United States

NLM ID

7506054

Subset

External Links

PubMed source DOI: 10.1007/BF01542794

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Formation of hybridoma clones in soft agarose: effect of pH and of medium.

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