A liquid ion-exchanger alternative to KCl for filling intracellular reference microelectrodes.

Pflugers Archiv : European journal of physiology ·Vol. 390 ·No. 1 ·1981-04-00 ·Pages 96-8

Thomas RC, Cohen CJ

Abstract

We have developed a filling solution for silanised microelectrodes consisting of potassium tetrakis (p-chlorophenyl) borate in octanol. Microelectrodes filled with this reference liquid ion-exchanger (RLIE) have equal selectivities to Na and K, and give the same membrane potential as do KCl-filled microelectrodes. The RLIE microelectrodes are more stable, less damaging to the cell membrane and do not leak Cl- ions. Their high resistance, however, makes them unsuitable for recording rapid potential changes or for passing current.

MeSH Terms

Animals Boron Compounds Membrane Potentials Microelectrodes Neurons/physiology Potassium Chloride Snails Tetraphenylborate/analogs & derivatives

Chemicals

Boron Compounds tetrakis(4-chlorophenyl)borate Tetraphenylborate Potassium Chloride

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Thomas R C

Cohen C J

References (8)

8 references, click to expand

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Neutral carrier ion-selective microelectrodes for measurement of intracellular free calcium.
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J Physiol. 1977 Dec;273(1):317-38 PMID: 23429
n-Alkanols potentiate sodium channel inactivation in squid giant axons.
Biophys J. 1979 Jun;26(3):585-90 PMID: 233577
A method of making fine double-barrelled potassium-sensitive micro-electrodes for intracellular recording [proceedings].
J Physiol. 1977 Aug;270(1):12P-14P PMID: 915764
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The specific ionic conductances and the ionic movements across the motoneuronal membrane that produce the inhibitory post-synaptic potential.
J Physiol. 1955 Nov 28;130(2):326-74 PMID: 13278905
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Article Info

Journal

Pflugers Archiv : European journal of physiology

Abbr.

Pflugers Arch

ISSN

0031-6768

DOI

10.1007/BF00582719

Published

1981-04-00

Pages

96-8

Language

English

Region

Germany

NLM ID

0154720

Subset

External Links

PubMed source DOI: 10.1007/BF00582719 View journal details

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A liquid ion-exchanger alternative to KCl for filling intracellular reference microelectrodes.

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