Home LiteratureArticle Details
PMID: 7198223 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The B goes to Z transition of poly(dG-dC) . poly(dG-dC) modified by some platinum derivatives.

Nucleic acids research ·Vol. 9 ·No. 21 ·1981-11-11 ·Pages 5659-69

Malfoy B, Hartmann B, Leng M

Abstract

Poly(dG-dC) . poly(dG-dC) was modified by chlorodiethylenetriamino platinum (II) chloride, cis-dichlorodiammine platinum (II) and trans-dichlorodiammine platinum (II), respectively. The conformation of these modified poly(dG-dC) . poly(dG-dC) was studied by circular dichroism. In 4 M Na+, the circular dichroism spectra of poly(dG-dC)dien-Pt (0 less than or equal to rb less than or equal to 0.2) are similar (rb is the amount of bound platinum per base). It is concluded that the conformation of these polymers belongs to the Z-family. Dien-Pt complexes stabilize the Z-form. The midpoint of the Z goes to B transition of poly(dG-dC)dien-Pt(0.12) is at 0.2 M NaCl. Moreover another B goes to Z transition is observed at lower salt concentration (midpoint at 6 mM NaCl). In 1 mM phosphate buffer, the stability of Z-poly(dG-dC)dien-Pt(0.12) is greatly affected by the presence of small amounts of EDTA. Poly(dG-dC) . poly(dG-dC) modified by cis-Pt and trans-Pt complexes do not adopt the Z-form even in high salt concentration.

MeSH Terms
Circular Dichroism Cisplatin Nucleic Acid Conformation Polydeoxyribonucleotides Structure-Activity Relationship
Chemicals
Polydeoxyribonucleotides poly(dG).poly(dC) Cisplatin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Malfoy B
Hartmann B
Leng M
References (24)
24 references, click to expand
  1. Resolution of alpha, beta and gamma DNA of Saccharomyces cerevisiae with the antitumor drug cis-Pt (NH3)2CL2. Evidence for preferential drug binding by GpG sequences of DNA.
    J Mol Biol. 1976 Jul 15;104(4):793-801 PMID: 785014
  2. Polymorphism of a synthetic DNA in solution.
    Nature. 1976 Mar 25;260(5549):365-6 PMID: 1256581
  3. A circular dichroism study of DNA.platinum complexes. Differentiation between monofunctional, cis-bidentate and trans-bidentate platinum fixation on a series of DNAs.
    Eur J Biochem. 1978 Feb;83(2):375-85 PMID: 631125
  4. "Alternating B-DNA" conformation for the oligo(dG-dC) duplex in high-salt solution.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2508-11 PMID: 288040
  5. Molecular structure of a left-handed double helical DNA fragment at atomic resolution.
    Nature. 1979 Dec 13;282(5740):680-6 PMID: 514347
  6. Left-handed DNA helices.
    Nature. 1980 Feb 21;283(5749):743-5 PMID: 7354864
  7. High-salt d(CpGpCpG), a left-handed Z' DNA double helix.
    Nature. 1980 Aug 7;286(5773):567-73 PMID: 7402336
  8. Salt-dependent dynamic structure of poly(dG-dC) x poly(dG-dC).
    Nature. 1980 Nov 27;288(5789):413-4 PMID: 6253836
  9. The tetramer d(CpGpCpG) crystallizes as a left-handed double helix.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4016-20 PMID: 6933447
  10. Conformation of poly(dG-dC) . poly(dG-dC) modified by the carcinogens N-acetoxy-N-acetyl-2-aminofluorene and N-hydroxy-N-2-aminofluorene.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4597-601 PMID: 6933507
  11. Left-handed double helical DNA: variations in the backbone conformation.
    Science. 1981 Jan 9;211(4478):171-6 PMID: 7444458
  12. Left-handed deoxyribonucleic acid double helix in solution.
    Biochemistry. 1981 Mar 31;20(7):2036-41 PMID: 7225372
  13. Conformational changes of poly(dG-dC) . poly(dG-dC) modified by the carcinogen N-acetoxy-N-acetyl-2-aminofluorene.
    Nucleic Acids Res. 1981 Mar 11;9(5):1241-50 PMID: 7232216
  14. Induction of the Z conformation in poly(dG-dC).poly(dG-dC) by binding of N-2-acetylaminofluorene to guanine residues.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1451-5 PMID: 6262801
  15. Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1619-23 PMID: 6262820
  16. Antiserum to Z-DNA.
    FEBS Lett. 1981 Sep 14;132(1):45-8 PMID: 6170528
  17. A study of chemically methylated deoxyribonucleic acid.
    Eur J Biochem. 1971 Jul 15;21(1):125-36 PMID: 5106032
  18. Modification of ribonucleic acid by chemical carcinogens. IV. Circular dichroism and proton magnetic resonance studies of oligonucleotides modified with N-2-acetylaminofluorene.
    J Mol Biol. 1971 Dec 14;62(2):331-46 PMID: 5138335
  19. Salt-induced co-operative conformational change of a synthetic DNA: equilibrium and kinetic studies with poly (dG-dC).
    J Mol Biol. 1972 Jun 28;67(3):375-96 PMID: 5045303
  20. Physical studies on deoxyribonucleic acid after covalent binding of a carcinogen.
    Biochemistry. 1972 Jul 4;11(14):2659-66 PMID: 4537912
  21. Persistence of O6-ethylguanine in rat-brain DNA: correlation with nervous system-specific carcinogenesis by ethylnitrosourea.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):639-43 PMID: 4522778
  22. Specific binding of antitumour drug cis-Pt(NH3)2C12 to DNA rich in guanine and cytosine.
    Nature. 1974 Oct 25;251(5477):736-7 PMID: 4427676
  23. Platinum complexes of nucleotides.
    Bioinorg Chem. 1975;5(1):51-8 PMID: 1182208
  24. Circular dichroism of mitomycin-DNA complexes. Evidence for a conformational change in DNA.
    Biochemistry. 1977 May 3;16(9):2040-6 PMID: 322710
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.21.5659
Published
1981-11-11
Pages
5659-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327550
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.21.5659 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2