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PMID: 7202001 Published · ppublish English Journal Article

Soluble aldehyde dehydrogenase and metabolism of aldehydes by soybean bacteroids.

Journal of bacteriology ·Vol. 151 ·No. 3 ·1982-09-00 ·Pages 1473-84

Peterson JB, LaRue TA

Abstract

A soluble aldehyde dehydrogenase (EC 1.2.1.3) was partially purified from Rhizobium japonicum bacteroids and from free-living R. japonicum 61A76. The enzyme was activated by NAD+, NADH, and dithiothreitol, and it reduced NAD(P)+. Acetaldehyde, propionaldehyde, butyraldehyde, benzaldehyde, and succinic semialdehyde were substrates. The Km for straight-chain aldehydes decreased with increasing carbon chain length. The aldehyde dehydrogenase was inhibited by 6-cyanopurine, but not by metronidazole. These compounds inhibited acetylene reduction, but not respiration, by isolated bacteroids.

MeSH Terms
Acetaldehyde/pharmacology Acetylene/metabolism Aldehyde Oxidoreductases/metabolism Aldehydes/metabolism Dithiothreitol/pharmacology Enzyme Activation Kinetics Metronidazole/pharmacology NAD/pharmacology Oxygen Consumption/drug effects Purines/pharmacology Rhizobium/enzymology,physiology Soybeans/microbiology
Chemicals
Aldehydes Purines NAD Metronidazole 6-cyanopurine Aldehyde Oxidoreductases Acetaldehyde Acetylene Dithiothreitol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peterson J B
LaRue T A
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.3.1473-1484.1982
Published
1982-09-00
Pages
1473-84
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220429
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.151.3.1473-1484.1982 PMC Full Text View journal details
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