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PMID: 7204491 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. I. Specimen handling techniques.

The Journal of cell biology ·Vol. 88 ·No. 2 ·1981-02-00 ·Pages 257-67

Saubermann AJ, Echlin P, Peters PD, Beeuwkes R

Abstract

X-ray microanalysis of frozen-hydrated tissue sections permits direct quantitative analysis of diffusible elements in defined cellular compartments. Because the sections are hydrated, elemental concentrations can be defined as wet-weight mass fractions. Use of these techniques should also permit determination of water fraction in cellular compartments. Reliable preparative techniques provide flat, smooth, 0.5 micrometers-thick sections with little elemental and morphological disruption. The specimen support and transfer system described permits hydrated sections to be transferred to the scanning electron microscope cold stage for examination and analysis without contamination or water loss and without introduction of extraneous x-ray radiation.

MeSH Terms
Animals Electron Probe Microanalysis/methods Elements Frozen Sections Liver/analysis,ultrastructure Mice Microscopy, Electron, Scanning Specimen Handling/methods
Chemicals
Elements
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Saubermann A J
Echlin P
Peters P D
Beeuwkes R
References (19)
19 references, click to expand
  1. Ultracryotomy: a technique for cutting ultrathin sections of unfixed frozen biological tissues for electron microscopy.
    J Microsc. 1970 Apr;91(2):105-17 PMID: 5468102
  2. Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver.
    J Cell Biol. 1971 Dec;51(3):772-804 PMID: 4942776
  3. Preparation of frozen hydrated tissue sections for x-ray microanalysis in the scanning electron microscope.
    Nature. 1974 Jan 11;247(5436):113-5 PMID: 4587972
  4. Instrumentation for direct microscopic elemental analysis of frozen biological tissue.
    Rev Sci Instrum. 1974 Feb;45(2):252-5 PMID: 4593631
  5. A cryostat approach to ultrathin "dry" frozen sections for electron microscopy: a morphological and x-ray analytical study.
    J Microsc. 1974 Jan;100(1):49-74 PMID: 4406569
  6. Preparing sections of skeletal muscle for transmission electron analytical microscopy (TEAM) of diffusible elements.
    J Microsc. 1975 Jan;103(1):101-12 PMID: 1173600
  7. The preparation, examination and analysis of frozen hydrated tissue sections by scanning transmission electron microscopy and x-ray microanalysis.
    J Microsc. 1975 Nov;105(2):155-91 PMID: 765465
  8. Quantitative electron probe microanalysis of biological thin sections: methods and validity.
    Ultramicroscopy. 1976 Sep-Oct;1(4):317-39 PMID: 1028201
  9. Elemental distribution in striated muscle and the effects of hypertonicity. Electron probe analysis of cryo sections.
    J Cell Biol. 1977 Sep;74(3):828-57 PMID: 302837
  10. Polymer cryoprotectants in the preservation of biological ultrastructure. I. Low temperature states of aqueous solutions of hydrophilic polymers.
    J Microsc. 1977 Aug;110(3):223-8 PMID: 73591
  11. Polymeric cryoprotectants in the preservation of biological ultrastructure. II. Physiological effects.
    J Microsc. 1977 Aug;110(3):239-55 PMID: 73592
  12. Polymeric cryoprotectants in the preservation of biological ultrastructure. III. Morphological aspects.
    J Microsc. 1977 Aug;110(3):257-70 PMID: 73593
  13. Cutting work in thick section cryomicrotomy.
    J Microsc. 1977 Sep;111(1):39-49 PMID: 606833
  14. Element concentration changes in mitotically active and postmitotic enterocytes. An x-ray microanalysis study.
    J Cell Biol. 1979 Feb;80(2):444-50 PMID: 457751
  15. Electron probe analysis of vascular smooth muscle. Composition of mitochondria, nuclei, and cytoplasm.
    J Cell Biol. 1979 May;81(2):316-35 PMID: 468907
  16. Biological X-ray microanalysis.
    J Microsc. 1979 Sep;117(1):145-63 PMID: 90731
  17. X-ray microanalysis of frozen hydrated tissue sections as a physiological tool.
    Microsc Acta Suppl. 1978;(2):130-41 PMID: 293470
  18. Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. II. Analysis of standard solutions and artificial electrolyte gradients.
    J Cell Biol. 1981 Feb;88(2):268-73 PMID: 7204492
  19. Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. III. Elemental content of cells in the rat renal papillary tip.
    J Cell Biol. 1981 Feb;88(2):274-80 PMID: 7204493
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.88.2.257
Published
1981-02-00
Pages
257-67
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111758
Subset
IM
Grants
NIADDK NIH HHS · AM 18249 · United States
NIGMS NIH HHS · GM 15904 · United States
NIADDK NIH HHS · RCDA AM 00224 · United States
External Links
PubMed source DOI: 10.1083/jcb.88.2.257 PMC Full Text View journal details
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