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PMID: 72075 Published · ppublish English Journal Article

Simplified silver-plating stain for flagella.

Journal of clinical microbiology ·Vol. 6 ·No. 4 ·1977-10-00 ·Pages 414-9

West M, Burdash NM, Freimuth F

Abstract

Rhodes' silver-plating technique for staining flagella was tested for its reliability and convenience as a routine procedure in the clinical laboratory. Modifications were made in the stain preparation and the procedure of staining and were tested with smears of known motile gram-negative nonfermentative bacilli. The stain has proved to be accurate and reliable and can be easily utilized with a minimum of training.

MeSH Terms
Alcaligenes/ultrastructure Bacteria/ultrastructure Bordetella/ultrastructure Evaluation Studies as Topic Flagella/ultrastructure Pseudomonas/ultrastructure Pseudomonas aeruginosa/ultrastructure Silver Species Specificity Staining and Labeling/methods
Chemicals
Silver
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
West M
Burdash N M
Freimuth F
References (6)
6 references, click to expand
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    J Gen Microbiol. 1958 Jun;18(3):639-48 PMID: 13549695
  2. Staining, shape and arrangement of bacterial flagella.
    J Bacteriol. 1951 Oct;62(4):377-89 PMID: 14897809
  3. Bacterial flagella staining.
    Lab Pract. 1972 Apr;21(4):267-8 PMID: 4118168
  4. Flagella staining.
    Zentralbl Bakteriol Orig A. 1973 Feb;223(2):240-3 PMID: 4123251
  5. Flagellation as a criterion for the classification of bacteria.
    Bacteriol Rev. 1965 Dec;29(4):442-65 PMID: 5322045
  6. A simplified Leifson flagella stain.
    J Clin Microbiol. 1976 Jun;3(6):632-4 PMID: 59736
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1977-10-00
Pages
414-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC274784
Subset
IM
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