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PMID: 7217207 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Appearance of fibronectin during the differentiation of cartilage, bone, and bone marrow.

The Journal of cell biology ·Vol. 88 ·No. 3 ·1981-03-00 ·Pages 630-6

Weiss RE, Reddi AH

Abstract

Fibronectin has been localized by indirect immunofluorescence during the various phases of endochondral bone formation in response to subcutaneously implanted demineralized bone matrix. Its histologic appearance has been correlated with results of biosynthetic experiments. (a) The implanted collagenous bone matrix was coated with fibronectin before and during mesenchymal cell proliferation. (b) During proliferation of mesenchymal precursor cells, the newly synthesized extracellular matrix exhibited a fibrillar network of fibronectin. (c) During cartilage differentiation, the fibronectin in the extracellular matrix was apparently masked by proteoglycans, as judged by hyaluronidase treatment. (d) Differentiating chondrocytes exhibited a uniform distribution of fibronectin. (e) Fibronectin was present in a cottony array around osteoblasts during osteogenesis. (f) The developing hematopoietic colonies revealed fibronectin associated with them. Therefore, it appears that fibronectin is ubiquitous throughout the development of endochondral bone and bone marrow.

MeSH Terms
Animals Bone Marrow Cells Cartilage/cytology,metabolism Cell Differentiation Fibronectins/metabolism Hematopoiesis Osteoblasts/metabolism Osteogenesis Rats Time Factors
Chemicals
Fibronectins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weiss R E
Reddi A H
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24 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.88.3.630
Published
1981-03-00
Pages
630-6
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112763
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.88.3.630 PMC Full Text View journal details
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