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PMID: 7232224 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Nucleic acids research ·Vol. 9 ·No. 7 ·1981-04-10 ·Pages 1691-706

Duckworth ML, Gait MJ, Goelet P, Hong GF, Singh M, Titmas RC

Abstract

Efficient mechanised synthesis of heptadecadeoxyribonucleotides has been achieved on an economically small scale by an improved solid phase phosphotriester method on a polydimethylacrylamide resin. Improvements were made in the preparation of dinucleotide building blocks, reaction conditions for oligonucleotide assembly and in purification of deprotected oligonucleotides by h.p.l.c. Several milligrams of pure heptadecamers were obtained. Two of the heptadecamers were designed for sequencing in opposite directions of DNA cloned in phage M13mp2.

MeSH Terms
Base Sequence Chromatography, High Pressure Liquid Indicators and Reagents Methods Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis
Chemicals
Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Duckworth M L
Gait M J
Goelet P
Hong G F
Singh M
Titmas R C
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.7.1691
Published
1981-04-10
Pages
1691-706
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326791
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.7.1691 PMC Full Text View journal details
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