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PMID: 7237132 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Radioautographic localization of prolactin messenger RNA on histological sections by in situ hybridization.

Brain research ·Vol. 211 ·No. 2 ·1981-05-04 ·Pages 433-8

Pochet R, Brocas H, Vassart G, Toubeau G, Seo H, Refetoff S, Dumont JE, Pasteels JL

Abstract

In situ hybridization of complementary [H3]DNA ([H3]cDNA) synthetized from purified rat prolactin messenger RNA (rPRL mRNA) was performed to specifically identify on histologic sections of rat hypophysis cells expressing the PRL gene. Radioautographic labelling occurred over weakly acidophilic cells, while other acidophils, with darker cytoplasm did not contain more silver grains than blood vessels.

MeSH Terms

Animals Autoradiography Cells, Cultured Estradiol/pharmacology Histocytochemistry Nucleic Acid Hybridization Pituitary Gland/metabolism Pituitary Gland, Anterior/metabolism Prolactin/genetics RNA, Messenger/genetics Rats Thyroidectomy Tritium

Chemicals

RNA, Messenger Tritium Estradiol Prolactin

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Pochet R

Brocas H

Vassart G

Toubeau G

Seo H

Refetoff S

Dumont J E

Pasteels J L

Article Info

Journal

Brain research

Abbr.

Brain Res

ISSN

0006-8993

DOI

10.1016/0006-8993(81)90969-0

Published

1981-05-04

Pages

433-8

Language

English

Region

Netherlands

NLM ID

0045503

Subset

External Links

PubMed source DOI: 10.1016/0006-8993(81)90969-0 View journal details

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Radioautographic localization of prolactin messenger RNA on histological sections by in situ hybridization.

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