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PMID: 7241652 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Tryptic peptide analysis of outer capsid polypeptides of mammalian reovirus serotypes 1, 2, and 3.

Journal of virology ·Vol. 38 ·No. 1 ·1981-04-00 ·Pages 208-18

Gentsch JR, Fields BN

Abstract

We studied the structural relationships among the outer capsid polypeptides of prototype strains of mammalian reovirus serotypes 1, 2, and 3 by tryptic peptide mapping. The micron1C polypeptide showed an extraordinary degree of conservation of its methionine-containing tryptic peptides. In contrast, the most abundant viral polypeptide, sigma 3, contained both conserved and unique methionine-containing tryptic peptides. The viral type-specific antigen, the sigma 1 polypeptide, contained both conserved and unique methionine- and tyrosine-containing tryptic peptides. These results suggested that the mammalian reovirus genome segments encoding each of the viral outer capsid polypeptides were derived from common ancestral segments which have diverged to different degrees.

MeSH Terms
Antigens, Viral/analysis Capsid/analysis,isolation & purification Hemagglutinins, Viral/analysis Peptide Fragments/analysis Reoviridae/analysis,immunology Trypsin Viral Proteins/analysis
Chemicals
Antigens, Viral Hemagglutinins, Viral Peptide Fragments Viral Proteins Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gentsch J R
Fields B N
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33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.1.208-218.1981
Published
1981-04-00
Pages
208-18
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171141
Subset
IM
Grants
NIAID NIH HHS · 1R01 AI-13178 · United States
NIAID NIH HHS · 5F32 AI-06O14-02 · United States
External Links
PubMed source DOI: 10.1128/JVI.38.1.208-218.1981 PMC Full Text View journal details
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